G150 is an inhibitor of cyclic GMP-AMP synthase (cGAS; IC50 = 10.2 nM for the human enzyme).1 It is selective for human cGAS over mouse cGAS (IC50 = 25,000 nM). G150 inhibits dsDNA-induced expression of IFNB1 in THP-1 cells and isolated human monocyte-derived macrophages (IC50s = 1.96 and 0.62 ?M, respectively) but not LPS-induced expresssion of TNF or poly(I:C)-induced rRNA degradation in the same cells when used at a concentration of 10 ?M.
1.Lama, L., Adura, C., Xie, W., et al.Development of human cGAS-specific small-molecule inhibitors for repression of dsDNA-triggered interferon expressionNat. Commun.10(1)2261(2019)