Fibronectin Adhesion-promoting Peptide (Trp-Glu-Pro-Pro-Arg-Ala-Arg-Ile) has medicinal interest as well as well-characterized structure. From a medical application point of view, this sequence (WQPPRARI) was found in the carboxy-terminated heparin-binding domain of fibronectin repeats III14. It is a potent inducer of stress fibers and focal adhesion in fibroblasts, which are involved in forming thrombosis related to diseases such as atherosclerotic cardiovascular disease.[2]
Fibronectin Adhesion-promoting Peptide has a low molecular weight of 1114.25 g/mol and lacks a secondary structure from a structural point of view. As such, irreversible adsorption based on surface-induced conformational changes is expected to be unimportant. Yet, this peptide provides a large enough structure for analysis by the BIAcore X SPR instrument, which has a detection limit of ～200 Da. Therefore, SPR was used to measure the adsorption properties of Fibronectin Adhesion-promoting Peptide on the polymer surface. SPR sensorgram of Fibronectin Adhesion-promoting Peptide adsorption on confined poly(2-vinylpyridine) surface were run at different concentrations of peptide (in mg/mL) in pH 7 HEPES buffer:? 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1.0, and 1.5, at T = 25 °C. The flow rate was 40 μL/min.[2]
Moreover, Fibronectin Adhesion-promoting Peptide directly promotes the adhesion, spreading, and migration of RCE cells in a concentration-dependent manner. [1]
References:
[1]. Mooradian, D L et al. Characterization of FN-C/H-V, a novel synthetic peptide from fibronectin that promotes rabbit corneal epithelial cell adhesion, spreading, and motility. Investigative ophthalmology & visual science vol. 34,1 (1993): 153-64.
[2]. Li, Xiao et al. Thermodynamic studies on the adsorption of Fibronectin Adhesion-promoting Peptide on nanothin films of poly(2-vinylpyridine) by SPR. Biomacromolecules vol. 5,3 (2004): 869-76.
纤连蛋白粘附促进肽 (Trp-Glu-Pro-Pro-Arg-Ala-Arg-Ile) 具有药用价值和良好表征的结构。从医学应用的角度来看，该序列 (WQPPRARI) 是在纤连蛋白重复序列 III14 的羧基端肝素结合域中发现的。它是成纤维细胞中应力纤维和粘着斑的有效诱导剂，参与形成与动脉粥样硬化性心血管疾病等疾病相关的血栓形成。[2]
Fibronectin Adhesion-promotion Peptide的分子量很低，为1114.25 g/mol，从结构上看没有二级结构。因此，基于表面诱导的构象变化的不可逆吸附预计不重要。然而，该肽提供了足够大的结构供 BIAcore X SPR 仪器分析，其检测限为 ~200 Da。因此，SPR被用来测量纤连蛋白粘附促进肽在聚合物表面的吸附性能。在 pH 7 HEPES 缓冲液中以不同浓度的肽（以 mg/mL 为单位）运行纤连蛋白粘附促进肽吸附在受限聚（2-乙烯基吡啶）表面上的 SPR 传感图:> 0.1、0.2、0.3、0.4、0.5、0.6、0.8 、1.0 和 1.5，在 T = 25 °C 时。流速为 40 μL/min。[2]
此外，Fibronectin Adhesion-promoting Peptide 以浓度依赖性方式直接促进 RCE 细胞的粘附、扩散和迁移。 [1]