GlpBio

Carvacrol

目录号: GC35612纯度: >99.50%同义词: 香芹酚
Carvacrol 是一种具有口服活性的单萜酚。
Carvacrol
Cas No.: 499-75-2
规格价格库存数量操作
100mg¥350.00现货
1
10mM (in 1mL DMSO)¥385.00现货
1
电话: 400-920-5774Email: sales@glpbio.cn

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产品描述 Description

Carvacrol is a monoterpene phenol with oral activity[1]. Carvacrol has antioxidant, antibacterial, antifungal, anticancer, anti-inflammatory, hepatoprotective, antispasmodic, and vasodilatory properties[2]. Carvacrol is usually used in research related to cancer and inflammation[3]. Carvacrol is also used as a flavoring agent and preservative in food at low concentrations, as well as a fragrance component in cosmetic formulas[4].

In vitro, treatment of MCF-7 breast cancer cells with Carvacrol (0-250µM; 24 or 48h) significantly reduced cell viability, induced cell cycle arrest at the G0/G1 phase, and promoted cell apoptosis[5].

In vivo, Carvacrol (20-80mg/kg/day; p.o.; 6 days) significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-6, malondialdehyde (MDA), nitric oxide (NO), and arginase activity in a rat sepsis model induced by LPS in a dose-dependent manner[6]. Carvacrol (30mg/kg/day; intraperitoneal injection from postoperative day 1 to day 9) significantly prolonged graft survival, reduced inflammatory cell infiltration in skin allografts, and decreased the expression of pro-inflammatory cytokines such as IL-6, TNF-α, and IL-17 in a mice skin allograft model[7].

References:
[1] Sharifi-Rad M, Varoni EM, Iriti M, et al. Carvacrol and human health: A comprehensive review. Phytother Res. 2018;32(9):1675-1687.
[2] Suntres ZE, Coccimiglio J, Alipour M. The bioactivity and toxicological actions of carvacrol. Crit Rev Food Sci Nutr. 2015;55(3):304-318.
[3] Singh J, Luqman S, Meena A. Carvacrol as a Prospective Regulator of Cancer Targets/Signalling Pathways. Curr Mol Pharmacol. 2023;16(5):542-558.
[4] Beninca T, Schmidt L, Thome Cardoso L, Rossini Augusti P, da Silva Malheiros P. Carvacrol as a food additive: Toxicological aspects and the role of nanotechnology in enhancing its antimicrobial and antioxidant properties. Food Res Int. 2024;197(Pt 1):115256.
[5] Mari A, Mani G, Nagabhishek SN, et al. Carvacrol Promotes Cell Cycle Arrest and Apoptosis through PI3K/AKT Signaling Pathway in MCF-7 Breast Cancer Cells. Chin J Integr Med. 2021;27(9):680-687.
[6] Kara M, Uslu S, Demirci F, Temel HE, Baydemir C. Supplemental carvacrol can reduce the severity of inflammation by influencing the production of mediators of inflammation. Inflammation. 2015;38(3):1020-1027.
[7] Zhao W, Tang H, Liang Z, et al. Carvacrol ameliorates skin allograft rejection through modulating macrophage polarization by activating the Wnt signalling pathway. Phytother Res. 2024;38(9):4675-4694.

Carvacrol 是一种具有口服活性的单萜酚[1]。Carvacrol具有抗氧化、抗菌、抗真菌、抗癌、抗炎、保肝、解痉和血管松弛剂特性[2]。Carvacrol通常用于癌症、炎症等相关研究[3]。Carvacrol 也被用作低浓度的食品调味成分和防腐剂，以及化妆品配方中的香味成分[4]。

体外实验中，用Carvacrol处理MCF-7乳腺癌细胞（0-250µM；24或48小时）显著降低细胞活性，诱导细胞周期停滞在G0/G1期，并促进细胞凋亡[5]。

体内实验中，Carvacrol（20-80mg/kg/天；口服；6天）剂量依赖性地降低了由LPS诱导的大鼠脓毒症模型中促炎细胞因子TNF-α和IL-6、丙二醛（MDA）、一氧化氮（NO）以及精氨酸酶活性的水平[6]。Carvacrol（30mg/kg/天；从术后第1天起至第9天进行腹腔注射）显著延长了皮肤异种移植模型小鼠的移植皮肤存活时间，减少了移植皮肤中的炎症细胞浸润，并降低了促炎细胞因子（如IL-6、TNF-α和IL-17）的表达[7]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:

Cell lines

MCF-7 cells

Preparation Method

MCF-7 cells were cultured in T-25 flasks containing 10% FBS with DMEM, 1% 100U/mL penicillin and 100μg/mL streptomycin and were maintained at 37℃ incubator with 5% CO2 with 95% humidity. The culture medium was routinely replenished with complete media twice a week. After attaining confl uence, the cells were trypsinized with Trypsin-EDTA solution. 1×104 MCF-7 cells per well were seeded in 96-well microplates for 24h. On achieving 70% confluence, cells were starved with an incomplete medium for 1h at 37℃. Post-starvation period, the cells were treated with different concentrations of carvacrol (0, 25, 50, 75, 100, 125, 150, 175, 200, 225, 250µM) for 24 and 48h. After treatment, MTT assays were used to detect cell viability. The cell cycle was analyzed by FACS. Annexin V-FITC/PI method was used for detection of cell apoptosis.

Reaction Conditions

0-250µM; 24 or 48h

Applications

Treatment of MCF-7 breast cancer cells with Carvacrol significantly reduced cell viability, induced cell cycle arrest at the G0/G1 phase, and promoted cell apoptosis.
Animal experiment [2]:

Animal models

Female Sprague–Dawley rats

Preparation Method

Forty-three adult female Sprague–Dawley rats weighting 200 to 300g were housed in polycarbonate cages in a room with controlled temperature (25±2°C), humidity (50±5%), and a 12h light/dark cycle. Animals had access to food and water ad libitum and were allowed to adapt to the local environment for 1 week before study. Feed and water were provided ad libitum. Rats were divided into six groups with regard to their body weights. Healthy Control Group (n=7): Rats in this group had access to food and water ad libitum during experimental period (7 days). Vehicle Control Group (n=7): 5% DMSO were administered by intragastric gavage in this group during 6 days. On the seventh day, 0.9% saline was injected to intraperitoneum (i.p.). Carvacrol Group I (n=6): 20mg/kg Carvacrol in 5% DMSO was administered to this group during 6 days, and on the seventh day, 1mg/kg LPS was injected i.p. Carvacrol Group II (n=8): 40mg/kg Carvacrol+1mg/kg LPS was administered to this group compatible with the same process of CVC group I. Carvacrol Group III (n=7): 80mg/kg Carvacrol+1mg/kg LPS was administered to this group compatible with same process of Carvacrol group I and II. Sepsis Group (LPS; n=8): Sublethal dose of E. coli LPS was given to rats intraperitoneal at 1mg/kg according to body weight and rats were killed at the indicated times following anesthetization. All tested solutions were administered orally (oral gavage) once a day at 9:00 a.m. for 6 days. Carvacrol and sepsis groups were treated with LPS on the seventh day. After 24h from LPS treatment, animals in all groups were sacrificed by exsanguination under anesthesia using a mixture of Ketamine/Xylazin. Serum samples were obtained by centrifuging the blood samples at 2,000×g for 10min. All of the samples were stored at -80°C until use. Two enzyme-linked immunosorbent assay kits were used for the TNF-α and the IL-6 assays. Malondialdehyde (MDA) is detected in quantitative employing the thiobarbituric acid assay. NO was measured as nitrite using the Griess reagent.

Dosage form

20-80mg/kg/day; p.o.; 6 days

Applications

Carvacrol significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-6, malondialdehyde (MDA) and nitric oxide (NO) in a rat sepsis model induced by LPS in a dose-dependent manner.

References:
[1] Mari A, Mani G, Nagabhishek SN, et al. Carvacrol Promotes Cell Cycle Arrest and Apoptosis through PI3K/AKT Signaling Pathway in MCF-7 Breast Cancer Cells. Chin J Integr Med. 2021;27(9):680-687.
[2] Kara M, Uslu S, Demirci F, Temel HE, Baydemir C. Supplemental carvacrol can reduce the severity of inflammation by influencing the production of mediators of inflammation. Inflammation. 2015;38(3):1020-1027.

产品文档 Product Documents

Purity:>99.50%Appearance:A liquid

化学性质Chemical Properties

CAS 号
499-75-2
同义词
香芹酚
SMILES
OC1=CC(C(C)C)=CC=C1C
分子式
C10H14O
分子量
150.22 g/mol
溶解性
Insoluble in Water; ≥28.1 mg/mL in EtOH; ≥28.8 mg/mL in DMSO
保存条件
Store at -20°C
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol