Z-Arg-Arg-AMC is a specific substrate for histone B with an Ex/Em of 360/460nm. Z-Arg-Arg-AMC has pH-dependent cleavage properties.At neutral pH, Z-Arg-Arg-AMC is readily cleaved by histone B. However, Z-Arg-Arg-AMC is very poorly active at acidic pH with only one-third of the activity at neutral pH [1-4].
References:
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Z-Arg-Arg-AMC是组织蛋白酶B的特异性底物，Ex/Em为360/460nm。Z-Arg-Arg-AMC具有pH依赖性的裂解特性，在中性pH下，Z-Arg-Arg-AMC很容易被组织蛋白酶B切割，但Z-Arg-Arg-AMC在酸性pH下的活性很差，只有中性pH下活性的三分之一[1-4]。