Boc-Lys-AMC is a dipeptide fluorescent substrate containing AMC (7-amino-4-methylcoumarin). Boc-Lys-AMC has poor enzyme cleavage kinetics and is mainly used as a deacetylated readout product in Histone DeACetylase (HDAC) fluorescence detection systems or as a fluorescent control substrate for some protease activity assays[1][2].
References:
[1] Wegener D, Wirsching F, Riester D, Schwienhorst A. A fluorogenic histone deacetylase assay well suited for high-throughput activity screening. Chem Biol. 2003;10(1):61-68.
[2] Ueki N, Wang W, Swenson C, McNaughton C, Sampson NS, Hayman MJ. Synthesis and Preclinical Evaluation of a Highly Improved Anticancer Prodrug Activated by Histone Deacetylases and Cathepsin L. Theranostics. 2016;6(6):808-816.
Boc-Lys-AMC是一种含有AMC（7-氨基-4-甲基香豆素）的二肽荧光底物。Boc-Lys-AMC被酶切割的动力学较差，主要用作组蛋白去乙酰化酶（HDAC）荧光检测系统中的去乙酰化读出产物，或作为一些蛋白酶活性测定的荧光对照底物[1][2]。