MeOSuc-Ala-Phe-Lys-AMC is a highly sensitive fluorescent substrate for plasmin. The excitation wavelength (λExc) of MeOSuc-Ala-Phe-Lys-AMC is 380 nm, and the emission wavelength (λEm) is 460 nm [1]. Plasmin is a protease that specifically degrades fibrin gels and is an important component of the fibrinolytic system. MeOSuc-Ala-Phe-Lys-AMC can be used for measuring plasmin activity [2]. The N-terminus of MeOSuc-Ala-Phe-Lys-AMC contains a methoxysuccinyl (MeOSuc) protecting group, which enhances its stability and specificity [3].
References:
[1] Le Dall J, Ho-Tin-Noé B, Louedec L, et al. Immaturity of microvessels in haemorrhagic plaques is associated with proteolytic degradation of angiogenic factors[J]. Cardiovascular research, 2010, 85(1): 184-193.
[2] Pierzchala P A, Dorn C P, Zimmerman M. A new fluorogenic substrate for plasmin[J]. Biochemical Journal, 1979, 183(3): 555-559.
[3] Cooley J, Takayama T K, Shapiro S D, et al. The serpin MNEI inhibits elastase-like and chymotrypsin-like serine proteases through efficient reactions at two active sites[J]. Biochemistry, 2001, 40(51): 15762-15770.
MeOSuc-Ala-Phe-Lys-AMC是一种高灵敏的纤溶酶荧光底物，MeOSuc-Ala-Phe-Lys-AMC的激发波长λExc=380nm，发射波长λEm=460nm[1]。纤溶酶（plasmin）是指能专一降解纤维蛋白凝胶的蛋白水解酶，是纤溶系统中的重要组份，MeOSuc-Ala-Phe-Lys-AMC可用于纤溶酶活性测定[2]。MeOSuc-Ala-Phe-Lys-AMC的N末端含有甲氧基琥珀酰（MeOSuc）保护基，这增强了其稳定性和特异性[3]。