H-D-Val-Leu-Lys-pNA . 2 HCl

H-D-Val-Leu-Lys-pNA . 2 HCl is a colorimetric substrate for the detection of protease activity, particularly plasmin ^[1]. H-D-Val-Leu-Lys-pNA . 2 HCl is catalytically bound and hydrolyzed by plasmin to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of plasminolytic activity^[2]. H-D-Val-Leu-Lys-pNA . 2 HCl is a colorimetric substrate for the detection of protease activity, particularly plasmin has a high affinity for purified hypersensitive granuloma (gPA) and can be used to analyze macrophage activation in allergic granuloma of mice ^[3]. H-D-Val-Leu-Lys-pNA . 2 HCl has been widely used to identify extracellular proteases from micromycete^[4].

References:
[1] Nagamatsu Y, Okamoto U, Tsuda Y, et al. Human leukocyte elastase-like proteinase purified by affinity chromatography with Suc-L-Tyr-D-Leu-D-Val-pNA, and its identification with human spleen fibrinolytic proteinase[J]. Thrombosis and haemostasis, 1984, 51(02): 243-247.
[2] Iwasaka M, Ueno S, Tsuda H. Effect of magnetic fields on the enzymatic activity of plasmin[C]//Proceedings of 16th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1994, 2: 762-763.
[3] Izaki S, Hibino T, Isozaki Y, et al. Plasminogen activator and plasminogen activator inhibitor associated with granulomatous inflammation: A study with murine leprosy[J]. Thrombosis and haemostasis, 1984, 52(06): 243-249.
[4] Stief T W, Richter A, Bünder R, et al. Functional Determination of Plasminin Arginine-stabilized Plasma[J]. Clinical and applied thrombosis/hemostasis, 2005, 11(3): 303-309.

H-D-Val-Leu-Lys-pNA . 2 HCl是一种用于检测蛋白酶活性的显色底物，尤其针对纤溶酶^[1]。H-D-Val-Leu-Lys-pNA . 2 HCl被纤溶酶催化结合并水解，释放对硝基苯胺（pNA），可在405nm处通过比色法检测，以此作为纤溶酶活性的衡量标准^[2]。H-D-Val-Leu-Lys-pNA . 2 HCl对纯化的高敏感性肉芽肿（gPA）具有高亲和力，可用于分析小鼠过敏性肉芽肿中的巨噬细胞活化^[3]。H-D-Val-Leu-Lys-pNA . 2 HCl已被广泛用于鉴定微型真菌的胞外蛋白酶^[4]。