Dansyl-Tyr-Val-Gly-OH is a substrate for peptidylglycine monooxygenase (PAM)[1]. PAM is a key enzyme that converts glycine-extended precursor peptides into biologically active α-amidated peptides, a process essential for the maturation and function of numerous peptide hormones and signaling peptides[2][3]. Dansyl-Tyr-Val-Gly-OH can be used for real-time monitoring of PAM enzyme activity and to elucidate the mechanism of peptide amidation in cell differentiation, metabolic regulation and tumorigenesis[4][5]. Dansyl-Tyr-Val-Gly-OH is widely used in fields such as oncology and biochemical analysis[6][7].
References:
[1] Welch EF, Rush KW, Arias RJ, Blackburn NJ. Pre-Steady-State Reactivity of Peptidylglycine Monooxygenase Implicates Ascorbate in Substrate Triggering of the Active Conformer. Biochemistry. 2022;61(8):665-677.
[2] Merkler DJ, Hawley AJ, Eipper BA, Mains RE. Peptidylglycine α-amidating monooxygenase as a therapeutic target or biomarker for human diseases. Br J Pharmacol. 2022;179(13):3306-3324.
[3] Bäck N, Mains RE, Eipper BA. PAM: diverse roles in neuroendocrine cells, cardiomyocytes, and green algae. FEBS J. 2022;289(15):4470-4496.
[4] Bauman AT, Jaron S, Yukl ET, Burchfiel JR, Blackburn NJ. pH Dependence of peptidylglycine monooxygenase. Mechanistic implications of Cu-methionine binding dynamics. Biochemistry. 2006;45(37):11140-11150.
[5] Bauman AT, Yukl ET, Alkevich K, McCormack AL, Blackburn NJ. The hydrogen peroxide reactivity of peptidylglycine monooxygenase supports a Cu(II)-superoxo catalytic intermediate. J Biol Chem. 2006;281(7):4190-4198.
[6] Miller DA, Sayad KU, Kulathila R, Beaudry GA, Merkler DJ, Bertelsen AH. Characterization of a bifunctional peptidylglycine alpha-amidating enzyme expressed in Chinese hamster ovary cells. Arch Biochem Biophys. 1992;298(2):380-388.
[7] Jones BN, Tamburini PP, Consalvo AP, et al. A fluorometric assay for peptidyl alpha-amidation activity using high-performance liquid chromatography. Anal Biochem. 1988;168(2):272-279.
Dansyl-Tyr-Val-Gly-OH 是肽基甘氨酸单加氧酶（PAM）的底物[1]。PAM 是一种关键酶，可将甘氨酸延伸的前体肽转化为具有生物活性的 α-酰胺化肽，这一过程对多种肽类激素和信号肽的成熟与功能至关重要[2][3]。Dansyl-Tyr-Val-Gly-OH 可用于实时监测 PAM 酶活性，并解析肽酰胺化在细胞分化、代谢调控及肿瘤发生中的作用机制[4][5]。Dansyl-Tyr-Val-Gly-OH 广泛应用于肿瘤学和生物化学分析等领域[6][7]。