Boc-Val-Leu-Gly-Arg-pNA is a chromogenic peptide substrate. Boc-Val-Leu-Gly-Arg-pNA can be specifically hydrolyzed by the limulus clotting enzyme, releasing p-nitroaniline (pNA) to produce a color change. Boc-Val-Leu-Gly-Arg-pNA can be used for quantitative detection of endotoxins and in research related to limulus amebocyte lysate (LAL) reagents[1-3].
References:
[1] Bahgat M, Aboul-Enein MN, El Azzouny AA, et al. A cyclohexanecarboxamide derivative with inhibitory effects on Schistosoma mansoni cercarial serine protease and penetration of mice skin by the parasite. Acta Pol Pharm. 2009 May-Jun;66(3):333-40.
[2] Bahgat M, Khalifa N. Differential inhibitory effect of newly synthesized pyridine-2-one derivatives on the cercarial serine protease activity of the parasite Schistosoma mansoni. Acta Pol Pharm. 2006 May-Jun;63(3):181-8.
[3] Iwanaga S, Morita T, Harada T, et al. Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins. Haemostasis. 1978;7(2-3):183-8.
Boc-Val-Leu-Gly-Arg-pNA是一种显色的多肽底物，Boc-Val-Leu-Gly-Arg-pNA可被鲎凝血酶特异性水解，释放出对硝基苯胺（pNA）以产生颜色变化。Boc-Val-Leu-Gly-Arg-pNA可用于内毒素定量检测和鲎试剂相关研究[1-3]。