Autoinducing Peptide I是由金黄色葡萄球菌分泌的一种群体感应自诱导肽。
Cas No.:200010-29-3
Sample solution is provided at 25 µL, 10mM.
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Autoinducing Peptide I is a quorum-sensing autoinducing peptide secreted by Staphylococcus aureus[1]. Autoinducing Peptide I acts as a specific agonist of the AgrC histidine kinase receptor and potently activates the agr system[2]. Upon binding and activating AgrC, Autoinducing Peptide I triggers the expression of virulence genes including RNAIII, and regulates the production of bacterial exotoxins, hemolysins, and other pathogenic factors[3]. Autoinducing Peptide I is widely used in the study of bacterial quorum sensing and virulence regulation mechanisms[4][5]. Autoinducing Peptide I is also used in studies of anti-infective mechanisms and novel antibacterial adjuvants, providing structural templates and target validation for the development of new antimicrobial therapies[6][7].
References:
[1] Lyon GJ, Wright JS, Muir TW, Novick RP. Key determinants of receptor activation in the agr autoinducing peptides of Staphylococcus aureus. Biochemistry. 2002;41(31):10095-10104.
[2] Wang B, Zhao A, Novick RP, Muir TW. Activation and inhibition of the receptor histidine kinase AgrC occurs through opposite helical transduction motions. Mol Cell. 2014;53(6):929-940.
[3] Gordon CP, Olson SD, Lister JL, Kavanaugh JS, Horswill AR. Truncated Autoinducing Peptides as Antagonists of Staphylococcus lugdunensis Quorum Sensing. J Med Chem. 2016;59(19):8879-8888.
[4] Arvidson S, Tegmark K. Regulation of virulence determinants in Staphylococcus aureus. Int J Med Microbiol. 2001;291(2):159-170.
[5] Tal-Gan Y, Stacy DM, Foegen MK, Koenig DW, Blackwell HE. Highly potent inhibitors of quorum sensing in Staphylococcus aureus revealed through a systematic synthetic study of the group-III autoinducing peptide. J Am Chem Soc. 2013;135(21):7869-7882.
[6] Daly SM, Joyner JA, Triplett KD, et al. VLP-based vaccine induces immune control of Staphylococcus aureus virulence regulation. Sci Rep. 2017;7(1):637.
[7] Tal-Gan Y, Ivancic M, Cornilescu G, Cornilescu CC, Blackwell HE. Structural characterization of native autoinducing peptides and abiotic analogues reveals key features essential for activation and inhibition of an AgrC quorum sensing receptor in Staphylococcus aureus. J Am Chem Soc. 2013;135(49):18436-18444.
Autoinducing Peptide I是由金黄色葡萄球菌分泌的一种群体感应自诱导肽[1]。Autoinducing Peptide I是AgrC组氨酸激酶受体的特异性激动剂，可高效激活agr系统[2]。Autoinducing Peptide I通过结合并激活AgrC，启动RNAⅢ等毒力基因的表达，从而调控细菌外毒素、溶血素等多种致病因子的产生[3]。Autoinducing Peptide I常用于细菌群体感应与毒力调控机制研究[4][5]。Autoinducing Peptide I也用于抗感染机制及新型抗菌佐剂的研究，为开发新型抗菌策略提供结构模板和靶点验证[6][7]。
| Cas No. | 200010-29-3 | SDF | |
| 分子式 | C43H60N8O13S2 | 分子量 | 961.11 |
| 溶解度 | DMSO : 100 mg/mL (104.05 mM; Need ultrasonic) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0405 mL | 5.2023 mL | 10.4046 mL |
| 5 mM | 208.1 μL | 1.0405 mL | 2.0809 mL |
| 10 mM | 104 μL | 520.2 μL | 1.0405 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet