Ac-Arg-pNA . HCl is a commonly used substrate for trypsin, which is utilized for inhibitor screening or enzyme concentration determination [1]. Ac-Arg-pNA . HCl is used to determine the activity of microbial trypsin-like enzymes[2]. Ac-Arg-pNA . HCl can be used to determine the activity of acylamino acid releasing enzyme (AARE)[3].
References:
[1] Kaiser-Alexnat R. Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte)[J]. Journal of invertebrate pathology, 2009, 100(3): 169-174.
[2] Nestler L, Evege E, McLaughlin J, et al. TrypLETM Express: a temperature stable replacement for animal trypsin in cell dissociation applications[J]. Quest, 2004, 1(1): 42-47.
[3] Gao R, Feng Y, Ishikawa K, et al. Cloning, purification and properties of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1[J]. Journal of Molecular Catalysis B: Enzymatic, 2003, 24: 1-8.
Ac-Arg-pNA . HCl是胰蛋白酶的一种常用底物，用于抑制剂筛选或酶浓度测定[1]。Ac-Arg-pNA . HCl被用于测定微生物胰蛋白酶样酶的活性[2]。Ac-Arg-pNA . HCl可用于测定酰基氨基酸释放酶（AARE）的活性[3]。