Fusidic acid-d6 is intended for use as an internal standard for the quantification of fusidic acid by GC- or LC-MS. Fusidic acid is a fusidane antibiotic originally isolated from F. coccineum.1 It is active against the Gram-positive bacteria S. aureus, S. pyogenes, C. diphtheriae, B. subtilis, and C. tetani (MIC50s = 0.01-20 µg/ml) but not the Gram-negative bacteria E. coli, S. typhimurium, and P. vulgaris or the fungi C. albicans and A. fumigatus (MIC50s = >100 µg/ml for all).2 Fusidic acid inhibits ribosomal recycling and protein translocation, processes mediated by elongation factor G (EF-G), in isolated E. coli ribosomes (IC50s = ~0.1 and ~200 µM, respectively).3 Topical administration of fusidic acid (2%) reduces the number of skin colony forming units (CFUs) and levels of TNF-α and IL-6 in a mouse model of methicillin-resistant S. aureus (MRSA) skin wound infection.4
1.Verbist, L.The antimicrobial activity of fusidic acidJ. Antimicrob. Chemother.25(Suppl. B)1-5(1990) 2.Godtfredsen, W.O., Jahnsen, S., Lorck, H., et al.Fusidic acid: A new antibioticNature193987(1962) 3.Savelsbergh, A., Rodnina, M.V., and Wintermeyer, W.Distinct functions of elongation factor G in ribosome recycling and translocationRNA15(5)772-780(2009) 4.Mohamed, M.F., and Seleem, M.N.Efficacy of short novel antimicrobial and anti-inflammatory peptides in a mouse model of methicillin-resistant Staphylococcus aureus (MRSA) skin infectionDrug Des. Devel. Ther.81979-1983(2014)