GlpBio

FITC-Dextran (MW 4000)

目录号: GC18871纯度: 不显示同义词: 荧光素异硫氰酸酯-葡聚糖
FITC-Dextran (MW 4000) (FD4)是一种大分子葡聚糖(MW ~4000)附着在异硫氰酸荧光素标签上，具有荧光性(Ex=495 nm; Em=525 nm) 。
FITC-Dextran (MW 4000)
Cas No.: 60842-46-8
规格价格库存数量操作
5mg¥490.00现货
1
10mg¥770.00现货
1
电话: 400-920-5774Email: sales@glpbio.cn

文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例
  • Nature cover
    Nature
    641, 529–536 (2025)
  • Nature cover
    Nature
    628, 630–638 (2024)
  • Nature cover
    Nature
    632, 686–694 (2024)
  • Nature cover
    Nature
    618, 1017–1023 (2023)
  • Nature cover
    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
  • Science cover
    Science
    387(6739) (2025)
  • Science cover
    Science
    387(6734) (2025)
  • Cell Research cover
    Cell Research
    35, 97–116 (2025)
  • Cell Research cover
    Cell Research
    34, 683–706 (2024)
  • Cell Research cover
    Cell Research
    33, 273–287 (2023)
  • Cell Research cover
    Cell Research
    33, 546–561 (2023)
  • Cell Research cover
    Cell Research
    33, 904–922 (2023)
  • Cell Research cover
    Cell Research
    31, 1291–1307 (2021)

产品描述 Description

FITC-Dextran (MW 4000) (FD4) is a large molecule of dextran (MW ~4000) attached to a fluorescein isothiocyanate tag, which makes it fluorescent (Ex=495 nm; Em=525 nm) [1-2].

FITC-Dextran (MW 4000) can be used for the study of cell permeability in bEnd.3 cells in vitro(0.1 mg/mL) and blood-brain barrier (BBB) permeability [3-4]. FITC-Dextran (MW 4000) can also be used as a marker to reveal cell damage caused by heat shock and to study the early and late stages of apoptosis[5].

FITC-Dextran (MW 4000) (0.6mg/g; i.g) can also be used to study intestinal barrier function [6].FITC-Dextran (MW 4000) (0.4mg/kg; i.p; 3days) can also be used to study blood-brain barrier permeability in male sprague dawley rats[7].

References:

[1]. Eriksson I, Öllinger K, et,al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. doi: 10.1007/978-1-4939-6934-0_11. PMID: 28456983.

[2]. Tsuneoka Y, Nishimura T, et,al. Fluorescein Permeability of the Blood-Brain Barrier Is Enhanced in Juvenile- but Not Young Adult-Onset Type 1 Diabetes in Rats. Biol Pharm Bull. 2021;44(8):1088-1092. doi: 10.1248/bpb.b21-00248. PMID: 34334494.

[3]. Wu S, Wang J et,al. Programmed cell death 10 increased blood-brain barrier permeability through HMGB1/TLR4 mediated downregulation of endothelial ZO-1 in glioblastoma. Cell Signal. 2023 Jul;107:110683. doi: 10.1016/j.cellsig.2023.110683. Epub 2023 Apr 17. PMID: 37075875.

[4]. Natarajan R, Northrop N et,al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. doi: 10.1002/cpns.25. PMID: 28398646; PMCID: PMC5470084.

[5]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

[6]. Yu W, Ou X, et,al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. doi: 10.1016/j.bbrc.2020.10.002. Epub 2020 Oct 21. PMID: 33097186; PMCID: PMC7576438.

[7]. Bommana MM, Kirthivasan B, et,al. In vivo brain microdialysis to evaluate FITC-dextran encapsulated immunopegylated nanoparticles. Drug Deliv. 2012 Aug;19(6):298-306. doi: 10.3109/10717544.2012.714812. Epub 2012 Aug 28. PMID: 22928708.

FITC-Dextran (MW 4000) (FD4)是一种大分子葡聚糖(MW ~4000)附着在异硫氰酸荧光素标签上，具有荧光性(Ex=495 nm; Em=525 nm) [1-2]。

FITC-Dextran (MW 4000) (0.1 mg/mL)可用于bEnd.3细胞通透性以及血脑屏障通透性研究[3-4]。FITC-Dextran (MW 4000)也可作为热休克细胞损伤的标志物，研究细胞凋亡的早期和晚期[5]。

FITC-Dextran (MW 4000) (0.6mg/g;i.g)也可用于研究肠屏障功能[6]。FITC-Dextran (MW 4000) (0.4mg/kg;i.p;3days)也可用于雄性大鼠血脑屏障通透性的研究[7]。

实验参考方法 Experimental Reference Method

FITC-Dextra体外血脑屏障通透性测定[1]:

体外血脑屏障通透性研究采用transwell法。

  1. 五万个 bEnd.3 cells细胞接种于孔径为0.4 μm的transwell系统中，放置于24孔板中。
  2. 在transwell单层形成后，将transwell和下腔的培养基分别替换为条件培养基和对照培养基，孵育过夜。
  3. 上腔加入FITC-Dextran(0.1 mg/mL)。孵育20分钟后检测荧光穿透。
  4. 血管完整性评价为transwell和下腔之间的吸光度差异。

细胞标记实验[2]:

适用于正在发生凋亡的HeLa细胞和人外周血单个核细胞(PBMC)。

1. 43.5℃孵育1h，37℃孵育8h，诱导细胞凋亡。

2. 将细胞悬浮在100 μL的培养基中，在Q-prep管与10 μL的propidium iodide (PI)，10 μL的FITC-Dextran (MW 4000)混合，PI和FITC-Dextran (MW 4000)的最终浓度分别为7.5 μM和1.13 μM。

3. 将细胞在室温黑暗条件下培养25分钟。

4. 取3 mL培养基的标记细胞，500 g离心10分钟。

5. 取离心细胞，加入1 mL培养基，用流式细胞术或荧光显微镜分析。

体内肠通透性试验方案[3]:
  1. 对肥胖和瘦弱小鼠肠道通透性的研究。小鼠按指定饮食喂养10-16周。

将小鼠体重分为两组，LBM剂量组和BW剂量组(瘦体重组和肥胖体重组)。

在研究结束时，小鼠为18-24周大。

2.    在研究当天，小鼠禁食4小时，然后按随机顺序口服FITC-Dextran (MW 4000) (溶解在浓度为125 mg/ml的PBS中)(600 mg/kg)。

3.    给药2小时后，用异氟烷麻醉小鼠(诱导5%，维持2%异氟烷，0.7 L/min N2O, 0.3 L/min O2)，在K3-EDTA-coated管中取眶后血，然后颈椎脱位。

4.    样品离心(4℃，7 min, 8000 g)，血浆收集于透明的Eppendorf管中。

5.   PBS给药小鼠的血浆用于标准曲线。用分光光度计分析血浆中FITC-Dextran浓度。

This protocol only provides a guideline, and should be modified according to your specific needs.

References:

[1]. Wu S, Wang J, et,al. Programmed cell death 10 increased blood-brain barrier permeability through HMGB1/TLR4 mediated downregulation of endothelial ZO-1 in glioblastoma. Cell Signal. 2023 Jul;107:110683. doi: 10.1016/j.cellsig.2023.110683. Epub 2023 Apr 17. PMID: 37075875.

[2]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

[3]. Voetmann LM, Rolin B, et al. The intestinal permeability marker FITC-dextran 4kDa should be dosed according to lean body mass in obese mice. Nutr Diabetes. 2023 Jan 5;13(1):1. doi: 10.1038/s41387-022-00230-2. PMID: 36604407; PMCID: PMC9816099.

产品文档 Product Documents

Purity:不显示

化学性质Chemical Properties

CAS 号
60842-46-8
同义词
荧光素异硫氰酸酯-葡聚糖
分子量
4000.00(Average) g/mol
溶解性
Water: 16mg/mL
保存条件
Store at 2-8°C, protect from light
General tips
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。
Shipping Condition
评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

计算工具摩尔浓度 / 稀释 / 分子量 / 单位换算 / 体内配方 / 溶解度

g/mol