Fibroblast Growth Factor-4 Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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FGF4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 182 amino acids and having a molecular mass of 19.8kDa.The FGF4 is purified by proprietary chromatographic techniques.
| Purity | > 95% as analyzed by SDS-PAGE, > 95% as analyzed by HPLC | Source | Escherichia Coli. |
| Phycical Appearance | White Freeze-dried Powder | Shipping Condition | Shipped at 2-8°C. |
| Synonyms | HBGF4; FGF-4; FGF4; KFGF; HSTF1. | ||
| Amino Acid Sequence | GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL. | ||
| Solubility | It is recommended to reconstitute the lyophilized FGF4 Human Recombinant sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Apparent Molecular Weight | ~19.4 kDa, on SDS-PAGE under reducing conditions. | ||
| Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. | ||
| Biological Activity | ED50 < 0.5 ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2.0 × 106 U/mg. | ||
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. | ||
| Formulation | Lyophilized after extensive dialysis against 50 mM HEPES, 750 mM NaCl, pH 7.5. | ||
FGF4 holds s comprehensive mitogenic and cell survival activities and takes part in a range of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF4 possess oncogenic transforming activity. FGF4 and FGF3, oncogenic growth factors are localized on chromosome 11. Co-amplification of both factors was found in several kinds of human tumors. FGF4 functions in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
ED50 < 0.5 ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2.0 × 106 U/mg.
Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.