Evans Blue tetrasodium salt

目录号: GC13947纯度: >98.00%同义词: 伊文思蓝; Direct Blue 53; C.I. 23860

Evans Blue tetrasodium salt是一种高效的囊泡谷氨酸吸收抑制剂（IC₅₀=87nM）。

Cas No.: 314-13-6

规格	价格	库存	数量	操作
100mg	¥350.00	现货	1
10mM (in 1mL DMSO)	¥385.00	现货	1

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Nature
641, 529–536 (2025)
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628, 630–638 (2024)
Nature
632, 686–694 (2024)
Nature
618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
Science
387(6739) (2025)
Science
387(6734) (2025)
Cell Research
35, 97–116 (2025)
Cell Research
34, 683–706 (2024)
Cell Research
33, 273–287 (2023)
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33, 546–561 (2023)
Cell Research
33, 904–922 (2023)
Cell Research
31, 1291–1307 (2021)

产品描述 Description

Evans Blue tetrasodium salt is a highly potent inhibitor of the vesicular glutamate uptake (IC₅₀=87nM)^[1]. Evans Blue is widely used in biomedicine including the estimation of blood volume, the assessment of vascular permeability to macromolecules, the detection of lymph nodes, and the location of the tumor lesions^[2].

Evans Blue (0-100μM, 1h) potently reduced Epsilon-toxin (ETX)-induced hemolysis (EC₅₀: ~1.2μM)^[3].

Evans Blue (2%, 4ml/kg; i.v.; 50min) combined with FITC-Dextran (2000kDa) can be used to quantify blood-brain barrier (BBB) leakage in specific regions of mice with lipopolysaccharide-induced BBB disruption and in apoE^-/- mice^[4].

References:
[1] Roseth S, Fykse EM, Fonnum F. Uptake of l‐glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. Journal of neurochemistry. 1995 Jul;65(1):96-103.
[2] Yao L, Xue X, Yu P, et al. Evans blue dye: a revisit of its applications in biomedicine. Contrast media & molecular imaging. 2018;2018(1):7628037.
[3] Gao J, Xin W, Huang J, et al. Hemolysis in human erythrocytes by Clostridium perfringens epsilon toxin requires activation of P2 receptors. Virulence. 2018 Dec 31;9(1):1601-14.
[4] Xu Y, He Q, Wang M, et al. Quantifying blood-brain-barrier leakage using a combination of evans blue and high molecular weight FITC-Dextran. Journal of neuroscience methods. 2019 Sep 1;325:108349.

Evans Blue tetrasodium salt是一种高效的囊泡谷氨酸吸收抑制剂（IC₅₀=87nM）^[1]。Evans Blue在生物医学领域有着广泛的应用，包括血容量的估计、血管对大分子通透性的评估、淋巴结的检测、肿瘤病变的定位等^[2]。

Evans Blue tetrasodium salt（0-100μM，1小时）可有效降低ε毒素（ETX）引起的溶血（EC₅₀：~1.2μM）^[3]。

Evans Blue（2%，4ml/kg；静脉注射；50分钟）与FITC-葡聚糖（2000kDa）结合可用于量化脂多糖诱导血脑屏障（BBB）破坏的小鼠和特定区域的血脑屏障渗漏的apoE^-/-小鼠^[4]。

实验参考方法 Experimental Reference Method

Cell experiment [1]:
Cell lines	Erythrocytes isolated from healthy volunteers
Preparation Method	Human blood was collected by venopuncture from healthy volunteers using evacuated blood collection tubes containing EDTA-2K. The blood of mice, rats, sheep, bovine, rabbits, monkeys, horses, dogs and goats were collected, then the erythrocytes were washed three times in 0.01M PBS (1,000g, 4°C, 5min). The “buffy coat” was removed and isolated erythrocytes were packed by centrifugation at 1,000g for 10min at 4°C. Washed erythrocytes were resuspended in 0.01M PBS to produce a 5% erythrocyte solution. The hemolytic activity was measured spectrophotometrically at 540nm. In the hemolytic assay, purified recombinant ETX (rETX) (0 ~ 33μM) and increasing concentrations of Evans Blue was added to the 5% red blood cell solution (final concentration of 3.3%) and incubated up to 1h at 37°C with constant swirling (300rpm). Then the 1.5ml tubes were centrifuged at 1,000g for 10min at 4°C, and the optical density at 540nm of the supernatants was determined as a measure of the released hemoglobin.
Reaction Conditions	0-100μM, 1h
Applications	Evans Blue potently reduced Epsilon-toxin (ETX)-induced hemolysis (EC₅₀: ~1.2μM).
Animal experiment [2]:
Animal models	Blood-brain barrier mouse model
Preparation Method	Eight C57BL/6J mice were intraperitoneally injected with 1mg/ml LPS (4ml/kg, diluted in normal saline) to induce BBB disruption. Another eight C57BL/6J mice were intraperitoneally injected with normal saline (4ml/kg) as control (LPS-control). Six hours after the injection, mice were anesthetized with 1.5% pentobarbital sodium (3ml/kg, i.p.), then cannulated with a 26 Ga catheter through the tail vein. 2% Evans Blue (4ml/kg, diluted in normal saline) was injected through the catheter. 50min after EB circulation, 6mg/ml FITC-Dextran (4ml/kg, diluted in Normal Saline) was injected through the catheter and circulated for 10min.
Dosage form	2%, 4ml/kg; i.v.; 50min
Applications	Evans Blue combined with FITC-Dextran (2000kDa) can be used to quantify blood-brain barrier (BBB) leakage in specific regions of mice with lipopolysaccharide-induced BBB disruption and in apoE^-/- mice.
References: [1] Gao J, Xin W, Huang J, et al. Hemolysis in human erythrocytes by Clostridium perfringens epsilon toxin requires activation of P2 receptors. Virulence. 2018 Dec 31;9(1):1601-14. [2] Xu Y, He Q, Wang M, et al. Quantifying blood-brain-barrier leakage using a combination of evans blue and high molecular weight FITC-Dextran. Journal of neuroscience methods. 2019 Sep 1;325:108349.

产品文档 Product Documents

批次：Purity:>98.00%

化学性质Chemical Properties

CAS 号

314-13-6

同义词

伊文思蓝; Direct Blue 53; C.I. 23860

化学名

sodium (6Z,6'Z)-6,6'-(2,2'-(3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(hydrazin-2-yl-1-ylidene))bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate)

SMILES

O=C1C(C(C=C/C1=N/NC(C(C)=C2)=CC=C2C3=CC=C(C(C)=C3)N/N=C4C(C(C(C=C\4)=C5S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C5)N)=O)=C6S([O-])(=O)=O)=C(C(S([O-])(=O)=O)=C6)N.[Na+].[Na+].[Na+].[Na+]

分子式

C₃₄H₂₄N₆Na₄O₁₄S₄

分子量

960.82 g/mol

溶解性

≥ 192.4 mg/mL in DMSO, ≥ 213.8 mg/mL in Water

保存条件

Store at RT

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

Shipping Condition

评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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