Eriodictyol

Cell experiment:

Primary cultures of cortical neurons are prepared from day 17 Sprague-Dawley rat embryos and maintained in Neurobasal A medium supplemented with B27. Experiments are performed 7 days after seeding. Aβ25-35 is dissolved in deionized distilled water at a concentration of 1 mM and incubated at 37°C for 7 days to induce its aggregation. Eriodictyol is added at indicated concentrations (20, 40, 80 μM) 2 h prior to Aβ25-35 treatment. Eriodictyol is dissolved in dimethyl sulfoxide (DMSO) at 64 mM as stock solution and diluted in culture medium before use[2].

Animal experiment:

Mice[1]Eighty female C57BL/6 mice are anesthetized by an intraperitoneal injection of 150 mg/kg ketamine HCl and 65 µg/kg xylazine hydrochloride. E. coli LPS is instilled intratracheally (25 µg in 50 µL sterile saline) during inspiration. The control mice receive PBS instillation, while the Eriodictyol- and vehicle-treated mice receive Eriodictyol (30 mg/kg, dissolved in PBS) and vehicle (PBS), respectively, orally 2 days prior to the induction of ALI. The mice are then sacrificed by an intravenous injection of thiopental 24 h after the induction of ALI. The thorax is opened and the blood is sampled by cardiac puncture. Simultaneously, three bronchoalveolar lavage (BAL) procedures are performed, each using 0.5 mL normal saline. The blood is centrifuged (2,000 × g, for 10 min at 4°C) and the serum is stored for further processing; the survival curve is then depicted using the Kaplan-Meier method[1].

References:

[1]. Zhu GF, et al. Eriodictyol, a plant flavonoid, attenuates LPS-induced acute lung injury through its antioxidative and anti-inflammatory activity. Exp Ther Med. 2015 Dec;10(6):2259-2266. Epub 2015 Oct 23.
[2]. Jing X, et al. Eriodictyol Attenuates β-Amyloid 25-35 Peptide-Induced Oxidative Cell Death in Primary Cultured Neurons by Activation of Nrf2. Neurochem Res. 2015 Jul;40(7):1463-71.