GlpBio

EGFP

目录号: GP23360纯度: >98%
COASDSData Sheet
Enhanced Green Fluorescent Protein Recombinant
EGFP
规格价格库存数量操作
5μg¥840.00现货
1
20μg¥2,030.00现货
1
1mg¥29,400.00现货
1
电话: 400-920-5774Email: sales@glpbio.cn

文献被引

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    Nature
    641, 529–536 (2025)
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    628, 630–638 (2024)
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    Nature
    632, 686–694 (2024)
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    Nature
    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
  • Cell cover
    Cell
    187(9):2288-2304 (2024)
  • Cell cover
    Cell
    183(7):1867-1883 (2020)
  • Science cover
    Science
    388(6745) (2025)
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    Science
    387(6739) (2025)
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    Science
    387(6734) (2025)
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    Cell Research
    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 273–287 (2023)
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    33, 546–561 (2023)
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    Cell Research
    33, 904–922 (2023)
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    Cell Research
    31, 1291–1307 (2021)

产品描述 Description

Recombinant EGFP produced in E.coli cells is a non-glycosylated, homodimeric protein containing 239 amino acid chain and having a molecular mass of 26.9kDa. Enhanced GFP (eGFP) has F64L and S65T mutations, which make GFP show increased fluorescence. EGFP is purified by proprietary chromatographic techniques.

产品文档 Product Documents

Purity:>98%
COASDSData Sheet

Product Data

Purity
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Shipping Condition
Shipped at Room temp.
Synonyms
Green fluorescent protein; GFP.
Amino Acid Sequence
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK
Solubility
It is recommended to reconstitute the lyophilized EGFP in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized EGFP although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution EGFP should be stored at 40C between 2-7 days and for future use below -180C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation
The EGFP was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4.