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Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton.The EGF is purified by proprietary chromatographic techniques.
在大肠杆菌中产生的人重组表皮生长因子是一种单一的非糖基化多肽链，包含 53 个氨基酸，分子量为 6222 道尔顿。EGF 通过专有色谱技术纯化。
| Purity | > 95% as analyzed by SDS-PAGE | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipping with dry ice. |
| Synonyms | Urogastrone; URG; EGF. | ||
| Amino Acid Sequence | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR. | ||
| Solubility | It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Apparent Molecular Weight | ~6.2 kDa, on SDS-PAGE under reducing conditions. | ||
| Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. | ||
| Biological Activity | ED50 < 0.6 ng/ml, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of > 1.7 × 106 U/mg. | ||
| Endotoxin Level | < 1 EU/μg of protein by gel clotting method | ||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. | ||
| Formulation | Lyophilized after extensive dialysis against 10 mM phosphate buffer, pH 7.0, 200 mM NaCl buffer. | ||
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
ED50 < 0.6 ng/ml, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of > 1.7 × 106 U/mg.
Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.