Dopamine HCl

Cell lines

Strial marginal cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

10 mM

Applications

In untreated normal strial marginal cells, the reaction product of Na-KATPase could be detectable. However, it became almost completely undetectable after an in vitro treatment with 10 mM Dopamine HCl. These results suggested that Dopamine HCl directly inhibited the Na-KATPase activity of strial marginal cells which might express dopamine receptors.

Animal models

SD rats

Dosage form

100 mg/kg; i.p.

Applications

In SD rats, Dopamine HCl treatment for 15 mins did not cause any reduction in degree of catatonia. The maximum score of Dopamine HCl treatment after 150 mins was only 3.5. It was suggested that Dopamine HCl did not cross the blood brain barrier and therefore, could not exert significant effect on drug-induced catatonia.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Kanoh N, Ogasawara H, Mohri D, Fukazawa K, Sakagami M. Cytochemical effects of in vitro dopamine treatment on the Na-KATPase activity in strial marginal cells. Acta Otolaryngol. 1996 Nov;116(6):824-7.

[2]. Jain NK, Rana AC, Jain SK. Brain drug delivery system bearing dopamine hydrochloride for effective management of parkinsonism. Drug Dev Ind Pharm. 1998 Jul;24(7):671-5.