DiI (DiIC18(3)) is a long-chain carbocyanine dye widely used as Di to label cells, organelles, liposomes, viruses and lipoproteins. DiI exhibits distinct orange fluorescence, facilitating multicolor imaging and flow cytometry analysis of live cells. DiI (DiIC18(3)) can be used as a fluorescent tracer of neurons for labeling cell bodies, axons, dendrites and dendritic spines, DiI (DiIC18(3)) was used as an anterograde tracer to study Neuronal development in vivo and used as a retrograde tracer to label hippocampal neuronal connections in fixed postmortem brain tissue[1]. Dil has also been used as a long-term plasma membrane marker in living cells[2].
DiI (DiIC18(3))是一种长链羰花青染料，被广泛用作Di来标记细胞，细胞器，脂质体，病毒和脂蛋白。DiI表现出明显的橙色荧光，从而促进活细胞的多色成像和流式细胞术分析。DiI (DiIC18(3))能够作为神经元的荧光示踪剂，用于标记细胞体、轴突、树突和树突棘，DiI (DiIC18(3))被用作顺行示踪剂来研究体内神经元发育，并用作逆行示踪剂来标记固定死后脑组织中的海马神经元连接[1]。Dil 也被用作活细胞中的长期质膜标记[2]。
References:
[1]. Mufson, E.J., Brady, D.R., and Kordower, J.H.Tracing neuronal connections in postmortem human hippocampal complex with the carbocyanine dye DiINeurobiol. Aging11(6)649-653(1990).
[2]. Honig, M.G., and Hume, R.I.Dil and diO: Versatile fluorescent dyes for neuronal labelling and pathway tracingTrends Neurosci.12(9)333-335(1989).