Digitonin is a steroidal saponin used extensively as a mild non-ionic detergent [1]. Digitonin finds application as a cholesterol precipitating agent [2]. Digitonin increases cell permeability by binding specifically to cholesterol in the cell membrane [4].
Digitonin had high exhibition against Caco-2, MCF-7 with IC50 of 15.72 ± 0.16 and 5.23 ± 0.61μM, respectively. Combinations of the panel of SM with a non-toxic concentration of digitonin (2 or 5 μM) generally enhanced the cytotoxicity in Caco-2, MCF-7 cells [3]. Pretreatment with digitonin increases the tumour uptake of carboplatin and potentiates the cytotoxic effect of carboplatin [4].
References:
[1]. Bridges C D. A method for preparing stable digitonin solutions for visual pigment extraction[J]. Vision Research, 1977, 17(2): 301-302.
[2]. Haust H L, Kuksis A, Beveridge J M R. Quantitative precipitation of various 3β-hydroxysterols with digitonin[J]. Canadian Journal of Biochemistry, 1966, 44(1): 119-128.
[3]. Eid S Y, El-Readi M Z, Wink M. Digitonin synergistically enhances the cytotoxicity of plant secondary metabolites in cancer cells[J]. Phytomedicine, 2012, 19(14): 1307-1314.
[4]. Lindnér P G, Heath D, Howell S B, et al. Digitonin enhances the efficacy of carboplatin in liver tumour after intra-arterial administration[J]. Cancer chemotherapy and pharmacology, 1997, 40(5): 444-448.
洋地黄皂苷是一种甾体皂苷,广泛用作温和的非离子洗涤剂[1]。洋地黄皂苷可用作胆固醇沉淀剂[2]。洋地黄皂苷通过特异性结合细胞膜中的胆固醇来增加细胞通透性[4]。
Digitonin 对 Caco-2、MCF-7 具有很强的抑制作用,IC50 分别为 15.72 ± 0.16 和 5.23 ± 0.61μM。 SM 组与无毒浓度的洋地黄皂苷（2 或 5 μM）的组合通常增强 Caco-2、MCF-7 细胞的细胞毒性[3]。毛地黄皂苷预处理可增加卡铂的肿瘤摄取并增强卡铂的细胞毒作用[4]。