Diadenosine pentaphosphate pentasodium是一种内源性的、具有血管活性的嘌呤二核苷酸，存在于血小板的分泌颗粒中，可激活5'-核苷酸酶以及抑制腺苷激酶活性。
Cas No.:4097-04-5
Sample solution is provided at 25 µL, 10mM.
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Diadenosine pentaphosphate pentasodium is an endogenous, vasoactive purine dinucleotide that is stored in platelet secretory granules. It can activate 5'-nucleotidase and inhibit adenosine kinase activity[1-2]. Diadenosine pentaphosphate pentasodium significantly increases the open probability of the ryanodine receptor (RyR2) channel and is commonly used in cancer-related studies[3-4].
In vitro, treatment of rat glomerular mesangial cells with Diadenosine pentaphosphate pentasodium (10μM) for 48 hours, Diadenosine pentaphosphate pentasodium significantly stimulated cell proliferation and protein synthesis by elevating cytosolic free calcium concentration ([Ca²⁺]i) and potentiated the proliferation induced by platelet-derived growth factor (PDGF)[5]. In cultured hippocampal neurons, treatment with Diadenosine pentaphosphate pentasodium (100μM) for 3 days, Diadenosine pentaphosphate pentasodium significantly inhibited total dendritic length and dendritic branch number through P2X1 receptor-mediated transient calcium influx in dendritic growth cones[6].
References:
[1] Bone R, Cheng YC, Wolfenden R. Inhibition of thymidine kinase by P1-(adenosine-5')-P5-(thymidine-5')-pentaphosphate. J Biol Chem. 1986 May 5;261(13):5731-5.
[2] Thore S, Wuttke A, Tengholm A. Rapid turnover of phosphatidylinositol-4,5-bisphosphate in insulin-secreting cells mediated by Ca2+ and the ATP-to-ADP ratio. Diabetes. 2007 Mar;56(3):818-26.
[3] Schulze DU, Düfer M, Wieringa B, et al. An adenylate kinase is involved in KATP channel regulation of mouse pancreatic beta cells. Diabetologia. 2007 Oct;50(10):2126-34.
[4] Eppstein DA, Schryver BB, Marsh YV. Stereoconfiguration markedly affects the biochemical and biological properties of phosphorothioate analogs of 2-5A core, (A2'p5')2A. J Biol Chem. 1986 May 5;261(13):5999-6003.
[5] Heidenreich S, Tepel M, Schlüter H, et al. Regulation of rat mesangial cell growth by diadenosine phosphates. J Clin Invest. 1995 Jun;95(6):2862-7.
[6] Diez-Zaera M, Ruiz-Calvo A, Diaz-Hernandez JI, et al. Diadenosine pentaphosphate regulates dendrite growth and number in cultured hippocampal neurons. Purinergic Signal. 2024 Apr;20(2):115-125.
Diadenosine pentaphosphate pentasodium是一种内源性的、具有血管活性的嘌呤二核苷酸，存在于血小板的分泌颗粒中，可激活5'-核苷酸酶以及抑制腺苷激酶活性[1-2]。Diadenosine pentaphosphate pentasodium可显著提高兰尼碱受体（RyR2）通道的开放概率，常被用于癌症的相关研究中[3-4]。
在体外，Diadenosine pentaphosphate pentasodium（10μM）处理大鼠肾小球系膜细胞48小时，Diadenosine pentaphosphate pentasodium通过升高胞质游离钙浓度（[Ca²⁺]i），显著刺激细胞增殖和蛋白质合成，并增强血小板衍生生长因子（PDGF）诱导的增殖效应[5]。在培养的海马神经元中，Diadenosine pentaphosphate pentasodium（100μM）处理3天，Diadenosine pentaphosphate pentasodium通过激活P2X1受体亚型介导的树突生长锥瞬时钙内流，显著抑制树突总长度和树突数量[6]。
| Cell experiment [1]: | |
Cell lines | Rat glomerular mesangial cells |
Preparation Method | Mesangial cells were isolated from 3-month-old male Wistar-Kyoto rats by serial sieving of minced renal cortical tissue from blood-free rat kidneys. Intact glomeruli without tubular contamination were digested in collagenase. Cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal calf serum, 100U/ml penicillin, and 100μg/ml streptomycin at 37°C in a humidified atmosphere of 95% air and 5% CO2. Glomerular mesangial cells treated with Diadenosine pentaphosphate pentasodium (10μM) for 48 hours. |
Reaction Conditions | 10μM; 48h |
Applications | Diadenosine pentaphosphate pentasodium significantly stimulated DNA synthesis and cell proliferation in rat mesangial cells. At concentrations of 10μM, Diadenosine pentaphosphate pentasodium induced an increase in MC proliferation. Diadenosine pentaphosphate pentasodium also stimulated protein synthesis but had no significant effects on RNA formation. Additionally, Diadenosine pentaphosphate pentasodium increased cytosolic free Ca2+ concentration ([Ca2+]i) and potentiated the effect of platelet-derived growth factor (PDGF) on MC growth, but did not potentiate the effect of insulin-like growth factor-1 (IGF-1). |
| Cas No. | 4097-04-5 | SDF | |
| 别名 | P1,P5-二(腺苷5′)五磷酸五钠盐 | ||
| 分子式 | C20H24N10Na5O22P5 | 分子量 | 1026.28 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 974.4 μL | 4.872 mL | 9.7439 mL |
| 5 mM | 194.9 μL | 974.4 μL | 1.9488 mL |
| 10 mM | 97.4 μL | 487.2 μL | 974.4 μL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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2.
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Quality Control & SDS
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- Purity: >96.00% Appearance: A solid
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