Deoxyadenosine 5-triphosphate is a deoxynucleotide triphosphate composed of an adenine base, a deoxyribose and three phosphate groups [1]. Deoxyadenosine 5‑triphosphate is one of the important “raw materials” for DNA polymerase to synthesize DNA chains in DNA replication or in vitro amplification [2]. Deoxyadenosine 5‑triphosphate regulates ribonucleotide reductase by binding to the active site, thereby participating in regulating the balance of deoxynucleoside triphosphate in cells [3].
In Escherichia coli, Deoxyadenosine 5‑triphosphate (400µM; 16h) stimulates bacterial biofilm formation [4].
References:
[1]. Reddy B S, Viswamitra M A. The molecular and crystal structure of the sodium salt of deoxyadenosine-5'-phosphate hexahydrate. Structural Science, 1975, 31(1): 19-26.
[2]. Houghton J A, Tillman D M, Harwood F G. Ratio of 2'-deoxyadenosine-5'-triphosphate/thymidine-5'-triphosphate influences the commitment of human colon carcinoma cells to thymineless death. Clinical cancer research: an official journal of the American Association for Cancer Research, 1995, 1(7): 723-730.
[3]. Alexandrova L A, Skoblov A Y, Jasko M V, et al. 2′-Deoxynucleoside 5′-triphosphates modified at α-, β-and γ-phosphates as substrates for DNA polymerases. Nucleic acids research, 1998, 26(3): 778-786.
[4]. Xi C, Wu J. dATP/ATP, a multifunctional nucleotide, stimulates bacterial cell lysis, extracellular DNA release and biofilm development. PloS one, 2010, 5(10): e13355.
Deoxyadenosine 5-triphosphate是一种由一个腺嘌呤碱基、一个脱氧核糖和三个磷酸基团组成的脱氧核苷酸三磷酸 [1]。Deoxyadenosine 5‑triphosphate是DNA聚合酶在DNA复制或体外扩增中合成DNA链的重要“原料”之一 [2]。Deoxyadenosine 5‑triphosphate通过结合活性位点来调节核苷酸还原酶，从而参与调节细胞内脱氧核苷三磷酸的平衡 [3]。
在大肠杆菌中，Deoxyadenosine 5‑triphosphate（400µM；16h）可刺激细菌生物膜的形成 [4]。