DC-05

目录号: GC33148纯度: >99.00%

A non-nucleoside DNMT1 inhibitor

Cas No.: 890643-16-0

规格	价格	库存	数量
1mg	¥1,043.00	现货	1
2mg	¥1,542.00	现货	1
5mg	¥2,520.00	现货	1
10mg	¥3,780.00	现货	1
10mM (in 1mL DMSO)	¥2,126.00	现货	1

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文献被引

本产品暂无引用记录;以下为 GlpBio 产品在 Nature / Cell / Science 等顶刊的客户引用样例

Nature
641, 529–536 (2025)
Nature
628, 630–638 (2024)
Nature
632, 686–694 (2024)
Nature
618, 1017–1023 (2023)
Nature
610, 366–372 (2022)
Cell
187(9):2288-2304 (2024)
Cell
183(7):1867-1883 (2020)
Science
388(6745) (2025)
Science
387(6739) (2025)
Science
387(6734) (2025)
Cell Research
35, 97–116 (2025)
Cell Research
34, 683–706 (2024)
Cell Research
33, 273–287 (2023)
Cell Research
33, 546–561 (2023)
Cell Research
33, 904–922 (2023)
Cell Research
31, 1291–1307 (2021)

产品描述 Description

DC-05 is an inhibitor of DNA methyltransferase 1 (DNMT1; IC₅₀ = 10.3 ?M).¹ It is selective for DNMT1 over DNMT3A and DNMT3B (IC₅₀s = >200 ?M), as well as the additional methyltransferases G9a, SUV39H1, MLL1, SET7/9, and PRMT1 (IC₅₀s = >150, >150, >150, >150, and 37.1 ?M, respectively). DC-05 (2.5-10 ?M) reduces proliferation of HCT116 colon cancer cells.

1.Chen, S., Wang, Y., Zhou, W., et al.Identifying novel selective non-nucleoside DNA methyltransferase 1 inhibitors through docking-based virtual screeningJ. Med. Chem.57(21)9028-9041(2014)

实验参考方法 Experimental Reference Method

Kinase experiment:

To measure the effects of DC-05 on mouse DNMT1 activity, 200 nM purified DNMT1 is incubated with 200 μM of DC-05 and S-adenosylmethionine (AdoMet) in the DNMT assay buffer in the assay plate at 37°C for 2 h. Next, every sample is incubated with the capture and detection antibody, followed by incubation with developer solution for 10 min at room temperature. The absorbance is measured at 450 nm using a microplate reader. S-Adenosylhomocysteine (AdoHcy) is used as a positive control[1].

References:

[1]. Chen S, et al. Identifying novel selective non-nucleoside DNA methyltransferase 1 inhibitors through docking-based virtual screening. J Med Chem. 2014 Nov 13;57(21):9028-41.

产品文档 Product Documents

Purity:>99.00%

化学性质Chemical Properties

CAS 号

890643-16-0

SMILES

OC(CNCCC1=CNC2=C1C=CC=C2)CN(C3=CC=CC=C34)C5=C4C=CC=C5

分子式

C₂₅H₂₅N₃O

分子量

383.49 g/mol

溶解性

DMSO : 100 mg/mL (260.76 mM);Water : < 0.1 mg/mL (insoluble)

保存条件

Store at -20°C

General tips

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
为了提高溶解度，请将管子加热至 37°C，然后在超声波浴中震荡一段时间。

Shipping Condition

评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备 RT，或根据请求配备蓝冰。

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