Cyclo(Arg-Gly-Asp-D-Phe-Cys) (Cyclo RGDfC) TFA, a cyclic RGD peptide which has high affinity to integrin interactions. Cyclo(Arg-Gly-Asp-D-Phe-Cys) TFA inhibits pluripotent marker expression in embryonic stem cells (ESCs) and the tumorigenic potential of mESCs in vivo. Cyclo(Arg-Gly-Asp-D-Phe-Cys) TFA can be used in the research of tumors[1].
Cyclo(Arg-Gly-Asp-D-Phe-Cys) TFA (0.5 mM; 24 h) 下调 mESC 的转录因子 Oct 4、Sox 2 和 Nanog [1]。
Cyclo(Arg-Gly-Asp-D-Phe-Cys) TFA (0.5 mM) 抑制整合素基因表达 mESC-col I (I 型胶原蛋白) 构建[1]。
Cyclo(Arg-Gly-Asp-D-Phe-Cys) TFA (0.5 mM) 处理的 mESC 形成团聚体并从表面脱离[1]。
Cyclo(Arg-Gly-Asp-D-Phe-Cys) TFA (0.5 mM, 24 h) 处理的 mESC 在存在或不存在白血病抑制因子 (LIF) 的情况下 (在双腿注射)，抑制严重联合免疫缺陷 (SCID) 小鼠产生畸胎瘤，这表明 mESC 肿瘤形成的过程在体内依赖于整合素相互作用[1]。
[1]. Hazenbiller O, et al. Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide. BMC Cell Biol. 2017 Nov 14;18(1):32.