Cyanine5.5 amine is a near-infrared fluorescent dye containing a free amino group that can be covalently coupled to biomolecules containing carboxyl, aldehyde or other reactive functionalities[1]. Cyanine5.5 amine is the amino derivative of Cyanine5.5, with an excitation wavelength of approximately 648-680nm and an emission wavelength of approximately 694-710nm[2]. Cyanine5.5 amine possesses a high molar extinction coefficient and bright far-red fluorescence, making it suitable for in vivo imaging and biomarker applications that require low background fluorescence[3]. Cyanine5.5 amine is insensitive to pH and exhibits good photostability and biocompatibility[4]. Cyanine5.5 amine is usually used for fluorescent labelling of biomolecules, preparation of nanoparticles, and small-animal in vivo imaging studies [5-7].
References:
[1] Zhang E, Zhukova V, Semyonkin A, et al. Release kinetics of fluorescent dyes from PLGA nanoparticles in retinal blood vessels: In vivo monitoring and ex vivo localization. Eur J Pharm Biopharm. 2020;150:131-142.
[2] Son Phan K, Nghi Do H, Thuy Doan B, et al. The Influence of Cyanine 5.5 and Doxorubicin on Cell Cycle Arrest, Magnetic Resonance, and Near-Infrared Fluorescence Optical Imaging for Fe3O4-Encapsulated PLA-TPGS Nanoparticles. ChemMedChem. 2025;20(5):e202400586.
[3] Konig SG, Kramer R. Accessing Structurally Diverse Near-Infrared Cyanine Dyes for Folate Receptor-Targeted Cancer Cell Staining. Chemistry. 2017;23(39):9306-9312
[4] Ebert B, Riefke B, Sukowski U, Licha K. Cyanine dyes as contrast agents for near-infrared imaging in vivo: acute tolerance, pharmacokinetics, and fluorescence imaging. J Biomed Opt. 2011;16(6):066003.
[5] Li L, Xue Y, Li X, et al. Copper Nanosheet-Based Wash-Free Fluorescence Imaging of Cancer Cells. Anal Chem. 2024;96(5):2052-2058.
[6] Luo J, Fan H, Shu C, et al. Immuno-protective cationic ginsenoside Rb1 vesicles for macrophage-mediated targeted therapy of inflammatory diseases. Mater Today Bio. 2025;34:102195.
[7] Mai L, Yao A, Li J, et al. Cyanine 5.5 conjugated nanobubbles as a tumor selective contrast agent for dual ultrasound-fluorescence imaging in a mouse model. PLoS One. 2013;8(4):e61224.
Cyanine5.5 amine是一种近红外荧光染料，含有游离氨基官能团，可用于与含羧基、醛基等官能团的生物分子进行共价偶联[1]。Cyanine5.5 amine是Cyanine5.5的氨基衍生物，激发波长约为648-680nm，发射波长约为694-710nm[2]。Cyanine5.5 amine具有高摩尔消光系数和明亮的远红外荧光特性，适用于需要低背景荧光的活体成像和生物标记应用[3]。Cyanine5.5 amine对pH不敏感，具有良好的光稳定性和生物相容性[4]。Cyanine5.5 amine通常用于生物分子的荧光标记、纳米粒子的制备以及小动物活体成像研究[5-7]。