Cyanine 5 Tyramide is a fluorescent signal amplification reagent formed by the chemical conjugation of the Cyanine5 fluorophore with tyramine (ex/em=645/670nm). In the presence of horseradish peroxidase (HRP) catalysis, the tyramine moiety of Cyanine 5 Tyramide is oxidized to generate highly reactive radicals, which covalently bind to tyrosine residues of proteins near the target site, thereby achieving significant signal amplification. Cyanine 5 Tyramide is used in bioimaging research such as immunohistochemistry (IHC), immunofluorescence (IF), and fluorescence in situ hybridization (FISH)[1].
References:
[1] Huang X, Wang J, Liu H, et al. Quantum dot-based FRET for sensitive determination of hydrogen peroxide and glucose using tyramide reaction. Talanta. 2013 Mar 15;106:79-84.
Cyanine 5 Tyramide是一种基于Cyanine5荧光染料与酪胺（tyramide）化学结合而成的荧光信号放大试剂（ex/em=645/670nm）。Cyanine 5 Tyramide在辣根过氧化物酶（HRP）催化下，其酪胺部分被氧化生成高活性自由基，与靶标附近蛋白质的酪氨酸残基共价结合，从而实现信号的高度放大。Cyanine 5 Tyramide可用于免疫组化（IHC）、免疫荧光（IF）、荧光原位杂交（FISH）等生物成像研究[1]。