Cy5 eGFP mRNA (5'CAP) is a green fluorescent protein mRNA transcribed in vitro by simulating mRNA processing in eukaryotes. Cy5 eGFP mRNA (5'CAP) carries Cy5 labeling(Cy5-UTP: UTP=3:1 (molar ratio)), Cap 1 cap structure, and poly (A) tail, which can effectively inhibit RNA mediated innate immune activation.
Green fluorescent protein eGFP is a fluorescent reporter gene commonly used in molecular biology research. Cy5 eGFP mRNA (5'CAP) can directly express proteins in the cytoplasm without relying on promoters, with a faster protein expression rate than transfected DNA. The protein expression level is directly related to the mRNA transfection level, and there is no risk of gene integration. After transfection of eGFP mRNA into cells, strong and bright green fluorescent protein can be expressed, with maximum excitation/emission wavelengths of 488/509nm, respectively. Cy5 is a commonly used cyanine fluorescent dye, with maximum excitation/emission wavelengths of 650/670nm, respectively.
Cy5 eGFP mRNA (5'CAP) can be used as a standard to detect the transfection efficiency of different transfection reagents, and can also be used as a control to study the transfection, localization, and expression of fluorescent proteins in mammalian cells.
Cy5 eGFP mRNA(5'CAP) 是通过模拟真核生物中mRNA加工过程在体外转录产生的绿色荧光蛋白mRNA，携带Cy5标记（Cy5-UTP：UTP=3:1（摩尔比））、Cap 1帽结构和poly(A)尾，能够有效抑制RNA介导的先天免疫激活。
绿色荧光蛋白eGFP是一种常用于分子生物学研究的荧光报告基因。Cy5 eGFP mRNA(5'CAP)能够在不依赖于启动子的情况下直接在细胞质中表达蛋白，蛋白表达速度比转染DNA更快，蛋白表达量与mRNA的转染量直接相关，并且没有基因整合的风险。eGFP mRNA转染细胞后能够表达强烈且明亮的绿色荧光蛋白，最大激发/发射光波长分别为488/509nm。Cy5是一种常用的花青类荧光染料，最大激发/发射光波长分别为650/670nm。
Cy5 eGFP mRNA(5'CAP)能够作为标准品检测不同转染试剂的转染效率，也能够作为对照研究哺乳动物细胞中荧光蛋白的转染、定位和表达。