Green fluorescent protein eGFP is a fluorescent reporter gene commonly used in molecular biology research. eGFP mRNA can directly express protein in the cytoplasm without relying on a promoter. The protein expression speed is faster than transfected DNA. The protein expression amount is directly related to the transfection amount of mRNA, and there is no risk of gene integration. Cells transfected with eGFP mRNA can express strong and bright green fluorescent protein eGFP, with excitation/emission wavelengths of 488 nm/509 nm respectively. eGFP mRNA can be used as a standard to test the transfection efficiency of different transfection reagents, and can also be used as a control to study the transfection and expression of fluorescent proteins in mammalian cells.
By simulating the mRNA processing process in eukaryotes, the product has a Cap 1 cap structure at the 5' end and a poly(A) tail at the 3' end, which increases the stability and translation efficiency of the mRNA[1].
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122
绿色荧光蛋白eGFP是一种常用于分子生物学研究的荧光报告基因。eGFP mRNA能够在不依赖于启动子的情况下直接在细胞质中表达蛋白,蛋白表达速度比转染DNA更快,蛋白表达量与mRNA的转染量直接相关,并且没有基因整合的风险。eGFP mRNA转染细胞后能够表达强烈且明亮的绿色荧光蛋白eGFP,激发/发射光波长分别为488 nm/509 nm。eGFP mRNA能够作为标准品检测不同转染试剂的转染效率,也能够作为对照研究哺乳动物细胞中荧光蛋白的转染和表达。
通过模拟真核生物中mRNA加工过程,该产品的5'端具有Cap 1帽结构,3'端具有poly(A)尾,增加了mRNA的稳定性和翻译效率[1]。