Cy5.5-NHS ester is a near-infrared amine reactive dye for labeling amino groups of peptides, proteins and oligonucleotides. Cy5.5-NHS ester is suitable for fluorescence analysis when the background fluorescence interferes. It can also be used for in vivo imaging experiments. Cy5.5-NHS ester can be dissolved in DMF and DMSO, the maximum excitation light and maximum emission light are around 675 nm and 693 nm, respectively.[1]
In vitro and in vivo experiment indicated that Cy5.5-NHS ester is a nice near-infrared fluorescent dye, which could be used for tumor targeting as well as examining the effect of D-glucose or L-glucose on 2-NBDG accumulation. Moreover, Cy5.5-NHS ester reacts swiftly that the tumor/muscle contrast could be clearly visualized from 30 min up to 24 h pi of 0.5 nmol Cy5.5-NHS. The tumor uptake of Cy5.5-NHS reached a maximum 30 minutes pi. [2]
References:
[1].Ju HR, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials. 2014. 35: 2302-2311.
[2].Zhen C. et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem. 2006 ; 17(3): 662–669.
Cy5.5-NHS 酯是一种近红外胺活性染料，用于标记肽、蛋白质和寡核苷酸的氨基。 Cy5.5-NHS ester 适用于背景荧光干扰时的荧光分析。它还可用于体内成像实验。 Cy5.5-NHS酯可溶于DMF和DMSO，最大激发光和最大发射光分别在675 nm和693 nm左右。[1]
体外和体内实验表明，Cy5.5-NHS酯是一种很好的近红外荧光染料，可用于肿瘤靶向以及检测D-葡萄糖或L-葡萄糖对2- NBDG积累。此外，Cy5.5-NHS 酯反应迅速，在 0.5 nmol Cy5.5-NHS 的感染后 30 分钟至 24 小时内，肿瘤/肌肉对比清晰可见。 Cy5.5-NHS 的肿瘤摄取在感染后 30 分钟达到最大值。 [2]