Cholic Acid-d4 is a tetradeuterium-labeled stable isotope derivative of cholic acid [1]. Cholic Acid-d4 is primarily used as a high-purity internal standard in mass spectrometry (LC‑MS/MS) and nuclear magnetic resonance (NMR) analysis to improve the accuracy and reproducibility of bile acid quantification—particularly when measuring endogenous bile acids in serum, plasma, bile, or feces [2-3].
References:
[1]. Koopman B J, Kuipers F, Bijleveld C M A, et al. Determination of cholic acid and chenodeoxycholic acid pool sizes and fractional turnover rates by means of stable isotope dilution technique, making use of deuterated cholic acid and chenodeoxycholic acid[J]. Clinica chimica acta, 1988, 175(2): 143-155.
[2]. Xu H, Xu Y, Zhao G, et al. The complete change in bile acids and steroids in systematic metabolomics applied to the intrahepatic cholestasis of pregnancy[J]. Molecular Omics, 2023, 19(5): 418-428.
[3]. Yang F, Takeuchi T, Tsuneyama K, et al. Experimental evidence of liver injury by BSEP-inhibiting drugs with a bile salt supplementation in rats[J]. Toxicological Sciences, 2019, 170(1): 95-108.
Cholic Acid-d4是胆酸的四氘标记稳定同位素衍生物 [1]。Cholic Acid-d4主要用作质谱（LC-MS/MS）和核磁共振（NMR）分析中的高纯度内标，以提高胆汁酸定量分析的准确性和可重复性，尤其是在测定血清、血浆、胆汁或粪便中的内源性胆汁酸时 [2-3]。