Ceramides Mixture is a main lipid component of the permeability barrier in the epidermis, where it plays a crucial role in maintaining skin integrity and barrier function. Ceramides Mixture is used mainly as a moisturizer in various cosmetic products. The concentration of Ceramides Mixture in healthy skin was found to be approximately 46-53% of total protein-bound lipids, whereas in affected areas of atopic dermatitis, this percentage decreased to 23-28%[1].
In vitro, Ceramides Mixture was grafted onto hyaluronic acid (HA) for local drug delivery in the skin. The HA-Ceramides Mixture derivatives (10-1000μg/mL) showed no obvious cytotoxicity to NIH-3T3 cells , and the cell survival rate was similar to that of the untreated control group. The THP-1 cell line was differentiated into macrophages, and the inhibitory effect of HA-Ceramides Mixture on LPS-induced IL-6 production was detected by ELISA. Macrophages differentiated from THP-1 monocytic human cell line co-incubated with LPS (1μg/mL) and HA-Ceramides Mixture derivatives (250 and 100μg/mL, respectively). HA-Ceramides Mixture exhibited a statistically significant inhibition effect on IL-6 production and LPS-induced inflammatory response. The highest anti-inflammatory effect was observed for the 250μg/mL concentration[2]. Ceramides Mixture are extracted from Korean traditional plants including P. ginseng, C. sinensis (a green tea plant), G. max seoritae (a Korean native soybean variety), G. max napjakong (a Korean native soybean variety), and C. japonica. Normal human epidermal keratinocytes (NHEKs) treated with above-mentioned Ceramides Mixture (0-4μg/mL or 0-10μg/mL) for 24h increased the expression of epidermal differentiation-related genes (such as FLG, CASP14 and INV). Ceramides Mixture significantly accelerated the recovery rate of the damaged skin barrier and enhanced the adhesion of the stratum corneum, especially C. sinensis Ceramides Mixture[3].
References:
[1] Macheleidt O, Kaiser H W, Sandhoff K. Deficiency of epidermal protein-bound omega-hydroxyceramides in atopic dermatitis. J Invest Dermatol. 2002 Jul;119(1):166-73.
[2] Juhaščik M, Štarmanová K, Brandejsová M, et al. Synthesis and self-assembling of hyaluronan grafted with ceramide NP for topical drug delivery. Carbohydr Polym. 2023 Dec 1:321:121283.
[3] Choi H K, Hwang K H, Hong Y D, et al. Ceramide NPs derived from natural oils of Korean traditional plants enhance skin barrier functions and stimulate expressions of genes for epidermal homeostasis. J Cosmet Dermatol. 2022 Oct;21(10):4931-4941.
Ceramides Mixture是表皮通透性屏障的主要脂质成分，在维持皮肤完整性和屏障功能方面起着至关重要的作用。Ceramides Mixture主要用作各种化妆品的保湿剂。Ceramides Mixture在健康皮肤中的浓度约为总蛋白结合脂质的46-53%，而在特应性皮炎的患处，这一百分比降至23-28%[1]。
在体外，Ceramides Mixture被移植到透明质酸（HA）上，HA-Ceramides Mixture衍生物（10-1000μg/mL）对NIH-3T3细胞无明显细胞毒性，细胞存活率与未处理对照组相近。LPS （1μg/mL）和HA-Ceramides Mixture（250μg/mL和100μg/mL）共孵育THP-1单核细胞系，诱导巨噬细胞分化。HA-Ceramides Mixture对IL-6的产生和LPS诱导的炎症反应有显著的抑制作用。以250μg/mL浓度抗炎作用最强[2]。从多种高丽传统植物中提取Ceramides Mixture，包括人参、绿茶、高丽大豆、高丽大豆、高丽大豆等，用上述几种Ceramides Mixture（0-4μg/mL或0-10μg/mL）处理24h后，正常人表皮角质形成细胞（NHEKs）的表皮分化相关基因（如FLG、CASP14和INV）表达增加。Ceramides Mixture能显著加快受损皮肤屏障的恢复速度，增强角质层的粘连，尤其是中华金盏花神经酰胺合剂[3]。