Cefoxitin is a cephalosporin antibiotic.1 It is active against numerous Gram-negative bacteria, including strains of P. mirabilis, P. vulgaris, E. coli, S. typhimurium, and S. enteritidis (MICs = 4-32 ?g/ml for all). It binds to B. subtilis penicillin-binding protein 2a (PBP2a), -2b, -3, -4, and -5 (IC50s = 0.72, 0.19, 1.04, 0.14, and 0.19 ?g/ml, respectively).2 Cefoxitin increases survival in mice infected with S. aureus, S. pyogenes, or K. pneumoniae (ED50s = 250, 63, and 795 ?g/animal, respectively).1 Formulations containing cefoxitin have been used in the treatment of bacterial infections.
1.Miller, A.K., Celozzi, E., Kong, Y., et al.Cefoxitin, a semisynthetic cephamycin antibiotic: In vivo evaluationAntimicrob. Agents Chemother.5(1)33-37(1974) 2.Sharifzadeh, S., Dempwolff, F., Kearns, D.B., et al.Harnessing β-lactam antibiotics for illumination of the activity of penicillin-binding proteins in Bacillus subtilisACS Chem. Biol.15(5)1242-1251(2020)