C20 Ceramide (d18:1/20:0) is a bioactive sphingolipid and a type of ceramide, containing a 20-carbon fatty acid chain (20:0) and an 18-carbon sphingosine backbone(d18:1).C20 Ceramide (d18:1/20:0) plays important roles in cellular signaling, particularly in apoptosis[1], cell cycle regulation, and stress responses[2].C20 Ceramide (d18:1/20:0) is commonly utilized in neurobiology research and cancer therpy[3], particularly in the study of its potential in Alzheimer's disease[4]. C20 Ceramide (d18:1/20:0) acts as a standard in quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry[5].
C20 Ceramide (d18:1/20:0) (2µM; 24h) treatment can induce apoptosis in primary cultured astrocytes from presenilin 1 (PS1) knock-in (PS1M146V) mice but not in wild-type astrocytes[6].
C20 Ceramide (d18:1/20:0) is elevated in presenilin 1 (PS1) knock-in (PS1M146V) mice brain[6].C20 Ceramide (d18:1/20:0) infusions of 100µM every other day for two weeks into the ventral hippocampus (VH) of adult male and female rats with implanted cannulas can trigger anhedonia-like behavior in the rats[7].
References:
[1]. Watts JD, Gu M, Patterson SD, Aebersold R, Polverino AJ. On the complexities of ceramide changes in cells undergoing apoptosis: lack of evidence for a second messenger function in apoptotic induction. Cell Death Differ. 1999 Feb;6(2):105-14. doi: 10.1038/sj.cdd.4400472. PMID: 10200556.
[2]. Kim HE, Grant AR, Simic MS, et al. Lipid Biosynthesis Coordinates a Mitochondrial-to-Cytosolic Stress Response. Cell. 2016 Sep 8;166(6):1539-1552.e16. doi: 10.1016/j.cell.2016.08.027. PMID: 27610574; PMCID: PMC5922983.
[3]. Sheridan M, Ogretmen B. The Role of Ceramide Metabolism and Signaling in the Regulation of Mitophagy and Cancer Therapy. Cancers (Basel). 2021 May 19;13(10):2475. doi: 10.3390/cancers13102475. PMID: 34069611; PMCID: PMC8161379.
[4]. Liu X, Jin Y, Cheng X, et al. The relevance between abnormally elevated serum ceramide and cognitive impairment in Alzheimer's disease model mice and its mechanism. Psychopharmacology (Berl). 2024 Mar;241(3):525-542. doi: 10.1007/s00213-024-06530-y. Epub 2024 Jan 26. PMID: 38277004.
[5]. Kasumov T, Huang H, Chung YM, et al. Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry. Anal Biochem. 2010 Jun 1;401(1):154-61. doi: 10.1016/j.ab.2010.02.023. Epub 2010 Feb 21. PMID: 20178771; PMCID: PMC2872137.
[6]. Wang G, Silva J, Dasgupta S, Bieberich E. Long-chain ceramide is elevated in presenilin 1 (PS1M146V) mouse brain and induces apoptosis in PS1 astrocytes. Glia. 2008 Mar;56(4):449-56. doi: 10.1002/glia.20626. PMID: 18205190.
[7]. Sambolín-Escobales L, Feliciano-Quiñones A, Tirado-Castro L, et al. Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats. Front Behav Neurosci. 2022 Aug 24;16:899627. doi: 10.3389/fnbeh.2022.899627. PMID: 36090653; PMCID: PMC9449580.
C20 Ceramide (d18:1/20:0)是一种生物活性鞘脂，也是神经酰胺的一种，含有20个碳的脂肪酸链（20:0）和18个碳的鞘氨醇骨架（d18:1）。C20 Ceramide (d18:1/20:0)在细胞信号传导中发挥重要作用，特别是在细胞凋亡[1]、细胞周期调控和应激反应中[2]。C20 Ceramide (d18:1/20:0)常用于神经生物学研究和癌症治疗[3]，尤其是研究其在阿尔茨海默病中的潜在作用[4]。C20 Ceramide (d18:1/20:0)可作为标准品，用于液相色谱电喷雾电离串联质谱法定量检测生物样本中神经酰胺[5]。
C20 Ceramide (d18:1/20:0)（2µM；24小时）处理可在早老素1（PS1）敲入（PS1M146V）小鼠来源的原代培养星形胶质细胞中诱导凋亡，但在野生型星形胶质细胞中则不能[6]。
在早老素1（PS1M146V）小鼠的大脑中，C20 Ceramide (d18:1/20:0)水平升高[6]。通过植入导管，连续两周每两天向成年雄性和雌性大鼠的腹侧海马（VH）中注入100µM的C20 Ceramide (d18:1/20:0)可在大鼠中引发类似快感缺失的行为[7]。