BSA-Palmitate Saturated Fatty Acid Complex is composed of palmitic acid (PA) and bovine serum albumin (BSA) in a molar ratio of approximately 6:1. It can be used to effectively deliver fatty acids (FA) to cells in culture to monitor fatty acid oxidation or similar processes in various cell metabolism studies[1-3]. BSA is one of the most abundant proteins in plasma and helps regulate the colloidal osmotic pressure of blood. It can be used in studies such as cell culture, immunochemistry, and protein quantification[4].
BSA/BSA-FA can be used for short-term cell culture applications (acute treatment up to 18h), but for long-term applications (more than 25h), the product should be filter-sterilized using a 0.2µm filter and sterile containers without affecting performance. For best results, it is recommended that this product be used in conjunction with the BSA Control of Fatty Acid Complex (Cat. No. GC46102).
References:
[1] Alsabeeh N, Chausse B, Kakimoto P A, et al. Cell culture models of fatty acid overload: Problems and solutions[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2018, 1863(2): 143-151.
[2] Wang D, Green M F, McDonnell E, et al. Oxygen flux analysis to understand the biological function of sirtuins[J]. Sirtuins: Methods and Protocols, 2013: 241-258.
[3] Bentebibel A, Sebastián D, Herrero L, et al. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation[J]. Biochemistry, 2006, 45(14): 4339-4350.
[4] Jin M, Zhu S, Hou Y. Insight on serum albumin: from structure and biological properties to functional biomaterials for bone repair[J]. ACS Biomaterials Science & Engineering, 2023, 9(5): 2235-2250.
BSA-Palmitate Saturated Fatty Acid Complex由棕榈酸（PA）和牛血清白蛋白（BSA）组成，棕榈酸：BSA的摩尔比约为6：1，可用于将脂肪酸（FA）有效递送至培养物中的细胞，以在各种细胞代谢研究中监测脂肪酸氧化或类似过程[1-3]。BSA是血浆中最丰富的蛋白质之一，有助于调节血液的胶体渗透压，能够应用于细胞培养、免疫化学和蛋白质定量等研究[4]。
BSA/BSA-FA可用于短期细胞培养应用（急性处理至18小时），但是对于长期应用（25小时以上），应该使用0.2µm过滤器和无菌容器对产品进行过滤灭菌，而不会影响性能。为获得最佳结果，建议本品与脂肪酸复合物的BSA对照品（目录号GC46102）联合使用。