Bromobimane (Monobromobimane)

Bromobimane (Monobromobimane) is a cell-permeable, thiol-reactive fluorescent probe with maximum absorption and emission wavelengths at 398nm and 490nm, respectively^{[1, 2, 3]}. Bromobimane (Monobromobimane) reacts rapidly with available sulfhydryl groups at physiological pH and produces a stable fluorescent signal^[4]. Bromobimane (Monobromobimane) can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H₂S, glutathione (GSH), proteins, and nucleotides^{[5, 6]}. Bromobimane (Monobromobimane) can be used to label GSH in liver sections on slides^[7].

References:
[1] Maeda K, Hägglund P, Finnie C, et al. Proteomics of disulphide and cysteine oxidoreduction[J]. Annual Plant Reviews Volume 28: Plant Proteomics, 2006: 71-97.
[2] Weber J, Bijol V, Wilke-Mounts S, et al. Cysteine-reactive fluorescence probes of catalytic sites of ATP synthase[J]. Archives of biochemistry and biophysics, 2002, 397(1): 1-10.
[3] Mohsin N Y, Demir H, Hadwan M H, et al. A New Fluorescent Method for Measuring Peroxiredoxin Enzyme Activity Using Monobromobimane[J]. Journal of Fluorescence, 2024: 1-9.
[4] Sardi F, Manta B, Portillo-Ledesma S, et al. Determination of acidity and nucleophilicity in thiols by reaction with monobromobimane and fluorescence detection[J]. Analytical Biochemistry, 2013, 435(1): 74-82.
[5] Głowacki R, Piechocka J, Bald E, et al. Application of separation techniques in analytics of biologically relevant sulfur compounds[M]//Handbook of Bioanalytics. Cham: Springer International Publishing, 2022: 1-24.
[6] Gilbert H T J, Mallikarjun V, Dobre O, et al. Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading[J]. Nature communications, 2019, 10(1): 4149.
[7] Wang H, Zhang R, Bridle K R, et al. Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury[J]. Scientific Reports, 2017, 7(1): 45374.

Bromobimane (Monobromobimane)是一种细胞可渗透的硫醇反应性荧光探针，最大吸收和发射波长分别为398nm和490nm^{[1, 2, 3]}。Bromobimane (Monobromobimane)能够在生理pH下与可用的巯基快速反应，并产生稳定的荧光信号^[4]。Bromobimane (Monobromobimane)可用于评估或定量各种含有活性硫或硫醇基团的化合物，包括H₂S、谷胱甘肽（GSH）、蛋白质和核苷酸^{[5, 6]}。Bromobimane (Monobromobimane)能够用于在载玻片上标记肝脏切片中的GSH^[7]。