Brilliant Black BN是一种偶氮染料和食品着色剂。
Cas No.:2519-30-4
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Brilliant black BN is an azo dye and a food colorant[1]. Brilliant Black BN is widely used in the food and cosmetics industries[2][3]. Brilliant Black BN also plays an key role in antiviral research, drug development, and biochemical studies[4][5].
In vitro, Brilliant Black BN (8.67, 86.7, and 867μg/ml; 24h) induced significant genotoxic effects in human peripheral blood lymphocytes and increased micronucleus formation and DNA damage[6].
In vivo, Brilliant Black BN (200mg/kg/day; intraperitoneal injection; 4 days) protected AG129 mice from lethal EV71 infection, reduced clinical scores, and significantly decreased viral titers in brain and muscle tissues[7].
References:
[1] Lang W, Sirisansaneeyakul S, Martins LO, et al. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench. J Environ Manage. 2014;132:155-164.
[2] Yamjala K, Nainar MS, Ramisetti NR. Methods for the analysis of azo dyes employed in food industry--A review. Food Chem. 2016;192:813-824.
[3] Joiner A, Philpotts CJ, Alonso C, Ashcroft AT, Sygrove NJ. A novel optical approach to achieving tooth whitening. J Dent. 2008;36 Suppl 1:S8-S14.
[4] Meng T, Wong SM, Chua KB. A Novel Attenuated Enterovirus A71 Mutant with VP1-V238A,K244R Exhibits Reduced Efficiency of Cell Entry/Exit and Augmented Binding Affinity to Sulfated Glycans. J Virol. 2021;95(22):e0105521.
[5] May LT, Briddon SJ, Hill SJ. Antagonist selective modulation of adenosine A1 and A3 receptor pharmacology by the food dye Brilliant Black BN: evidence for allosteric interactions. Mol Pharmacol. 2010;77(4):678-686.
[6] Macioszek VK, Kononowicz AK. The evaluation of the genotoxicity of two commonly used food colors: Quinoline Yellow (E 104) and Brilliant Black BN (E 151). Cell Mol Biol Lett. 2004;9(1):107-122.
[7] Meng T, Jia Q, Wong SM, Chua KB. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN. J Virol. 2019;93(17):e00061-19.
Brilliant Black BN是一种偶氮染料和食品着色剂[1]。Brilliant Black BN 广泛应用于食品和化妆品行业[2][3]。Brilliant Black BN 在抗病毒研究、药物开发和生化研究中也发挥着关键作用[4][5]。
体外实验中，Brilliant Black BN（8.67、86.7 和 867μg/ml；24小时）在人外周血淋巴细胞中诱导了显著的遗传毒性效应，增加了微核形成和DNA损伤[6]。
体内实验中，Brilliant Black BN（200mg/kg/天；腹腔注射；4天）保护了AG129小鼠免于致死性EV71感染，降低了临床评分，并显著降低了脑组织和肌肉组织中的病毒滴度[7]。
| Cell experiment [1]: | |
Cell lines | Human peripheral blood lymphocytes |
Preparation Method | Heparinized peripheral venous blood samples were collected from healthy, non-smoking donors and processed for the MN and Comet assays. A negative control (growth medium) was included in each experiment. DEB-a metabolite of 3,5-butadiene, and a mutagenic and carcinogenic agent at the concentration 0.09μg/ml was used as a positive control to obtain a reference for the effects induced by the tested chemicals. Whole blood samples (72h culture) were incubated in RPMI 1640 medium supplemented with 20% bovine serum, LF-7 (substance stimulating lymphocytes division), penicillin (100U/ml) and streptomycin (0.1mg/ml) at 37°C. Lymphocytes were treated with Brilliant Black BN (8.67, 86.7, and 867μg/ml) for 24h, centrifuged (1500rpm, 5min) and resuspended in fresh medium. Cytochalasine B (6μg/ml) was added 44h after LF-7 stimulation. After a total of 72h incubation, the cultures were harvested and treated with hypotonic solution (0.075M KCl) to lyse red blood cells. For the fixation, cold, freshly prepared fixative, a mixture of methanol:acetic acid (3:1, v/v) was used. Lymphocytes were spread onto microscopic slides and stained with acridine orange (2μg/ml) or Giemsa dye. A nuclear division index (NDI) was calculated as follows: NDI=(M1+2×M2+3×M3+4×M4)/N, where M1-M4 respectively represent the number of cells with one to four nuclei and N is the total number of cells scored. The frequency of MN induction was evaluated by scoring 4000 binucleated lymphocytes (1000 cells per slide) per sample. |
Reaction Conditions | 8.67, 86.7, and 867μg/ml; 24h |
Applications | Brilliant Black BN (8.67, 86.7, and 867μg/ml; 24h) induced significant increased micronucleus formation in human peripheral blood lymphocytes. |
| Animal experiment [2]: | |
Animal models | AG129 mice |
Preparation Method | Eight 14-day-old AG129 mouse neonates in each group were challenged with 10 LD50 of EV71-B4 (3×1010 TCID50) or EV71-C1 (1.5×108 TCID50) in 0.2ml of PBS via the intraperitoneal (i.p.) route. From 0 to 3 days post challenge, the mice in the Brilliant Black BN protection group were administered 1 dose daily of Brilliant Black BN in PBS at 200mg/kg through the i.p. route, while only PBS was used in the mock treatment group. The mice were monitored at a frequency of 24h for clinical illness until 21 days post challenge. Clinical illness was scored as follows: 0, normalcy; 1, ruffled hair and hunchbacked appearance; 2, lethargy with limb weakness; 3, paralysis in one limb; 4, paralysis in two limbs but with ability to move and ingest; 5, immobility or unconsciousness; 6, death. As mice with a clinical score of 5 usually die in 1 day, they were euthanized with carbon dioxide to reduce their suffering and their death days were accounted on the next day. At the end of the experiments, all mice were euthanized with carbon dioxide. To study viral propagation in the EV71-C1-challenged mice, the muscles of hind limbs and the brains (5 pups per group) were harvested, weighed, and stored at -80°C after euthanasia with carbon dioxide at 4, 8, and 12 days post challenge. The samples were homogenized at 500mg/ml in DMEM with 10% FBS and 5×antibioticantimycotic by using a TissueLyser LT homogenizer. The homogenates were frozen-thawed twice and then kept at 4°C for 1h. The titers of virus progeny in the supernatants of clarified homogenates (10,000×g for 10min at 4°C) were determined in RD cells. |
Dosage form | 200mg/kg/day; intraperitoneal injection; 4 days |
Applications | Brilliant Black BN protected AG129 mice from lethal EV71 infection, reduced clinical scores, and significantly decreased viral titers in brain and muscle tissues. |
References: | |
| Cas No. | 2519-30-4 | SDF | |
| 别名 | 食品黑1,E 151 | ||
| Canonical SMILES | O=S(C1=C2C=C(S(=O)(O[Na])=O)C(/N=N/C3=C4C=C(S(=O)(O[Na])=O)C=CC4=C(/N=N/C5=CC=C(S(=O)(O[Na])=O)C=C5)C=C3)=C(O)C2=C(NC(C)=O)C=C1)(O[Na])=O | ||
| 分子式 | C28H17N5Na4O14S4 | 分子量 | 867.68 |
| 溶解度 | DMSO: 125 mg/mL (144.06 mM) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1525 mL | 5.7625 mL | 11.525 mL |
| 5 mM | 230.5 μL | 1.1525 mL | 2.305 mL |
| 10 mM | 115.2 μL | 576.2 μL | 1.1525 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >60.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet