BODIPY-aminoacetaldehyde diethyl acetal (BAAA-DA) is a stable precursor of BODIPY-aminoacetaldehyde (BAAA), a cell-permeable fluorescent substrate for aldehyde dehydrogenase (ALDH) with an Ex/Em of 488/512nm[1-4].
BODIPY-aminoacetaldehyde diethyl acetal converts to BAAA in acidic conditions. BAAA is uncharged and can diffuse freely across the plasma membrane of intact living cells with cellular permeability. BAAA is converted into Bodipy-amino acetic acid (BAA) intracellular by ALDH, which is retained by cells due to the net negative charge of BAA and can be used to identify cells with high ALDH activity [1-2]. BODIPY-aminoacetaldehyde diethyl acetal has been used to isolate human hematopoietic progenitor cells, which have high ALDH activity, and via flow cytometry to sort cancer stem cells that contain high levels of ALDH[1，3]. BODIPY-aminoacetaldehyde diethyl acetal can be used to characterize live ALDHpos cells, and the BAA used in the cells can be excited at 488nm with a maximum emission wavelength of 512nm[4].
References:
[1] Storms R W, Trujillo A P, Springer J B, et al. Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity[J]. Proceedings of the National Academy of Sciences, 1999, 96(16): 9118-9123.
[2] Moreb J S, Zucali J R, Ostmark B, et al. Heterogeneity of aldehyde dehydrogenase expression in lung cancer cell lines is revealed by Aldefluor flow cytometry‐based assay[J]. Cytometry Part B: Clinical Cytometry: The Journal of the International Society for Analytical Cytology, 2007, 72(4): 281-289.
[3] Leng Z, Yang Z, Li L, et al. A reliable method for the sorting and identification of ALDHhigh cancer stem cells by flow cytometry[J]. Experimental and Therapeutic Medicine, 2017, 14(4): 2801-2808.
[4] Pomper M G, Wang H, Minn I, et al. Red fluorescent aldehyde dehydrogenase (ALDH) substrate: U.S. Patent 10,156,521[P]. 2018-12-18.
BODIPY-aminoacetaldehyde diethyl acetal（BAAA-DA）是BODIPY-氨基乙醛（BAAA）的稳定前体，BAAA是醛脱氢酶（ALDH）的细胞渗透性荧光底物，Ex/Em为488/512nm[1-4]。
BODIPY-aminoacetaldehyde diethyl acetal在酸性条件下转化为BAAA。BAAA不带电荷，可以在完整活细胞的质膜上自由扩散，具有细胞渗透性。BAAA通过ALDH在细胞内转化为BODIPY-氨基乙酸（BAA），由于BAA净负电荷，BAA被细胞保留并可用于识别具有高ALDH活性的细胞[1-2]。BODIPY-aminoacetaldehyde diethyl acetal已用于分离具有高ALDH活性的人造血祖细胞，并通过流式细胞术分选含有高水平ALDH的癌症干细胞[1，3]。BODIPY-aminoacetaldehyde diethyl acetal可用于鉴定活ALDHpos细胞，可以在488nm处激发，最大发射波长为512nm[4]。