BODIPY 503/512 is a lipophilic, amine-reactive fluorescent probe.1 It has been used to label oligonucleotide probes and primers for the quantitation of DNA and RNA by PCR and to monitor the uptake and trafficking of BODIPY-labeled proteins and other compounds within cells by fluorescence microscopy.2,3,4,1 BODIPY 503/512 has also been used to identify and sort adipocytes from mouse white and brown adipose tissue by flow cytometry.5 It displays excitation/emission maxima of 503/512 nm, respectively.1
|1. Murakami, M., Cabral, H., Matsumoto, Y., et al. Improving drug potency and efficacy by nanocarrier-mediated subcellular targeting. Sci. Transl. Med. 3(64), 64ra62 (2011).|2. Kurata, S., Kanagawa, T., Yamada, K., et al. Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY• FL-labeled probe or primer. Nucleic Acids Res. 29(6), E34 (2001).|3. Bittel, A.M., Saldivar, I.S., Dolman, N.J., et al. Superresolution microscopy with novel BODIPY-based fluorophores. PLoS One 13(10), e0206104 (2018).|4. Surewaard, B.G.J., Deniset, J.F., Zemp, F.J., et al. Identification and treatment of the Staphylococcus aureus reservoir in vivo. J. Exp. Med. 213(7), 1141-1151 (2016).|5. Hagberg, C.E., Li, Q., Kutschke, M., et al. Flow cytometry of mouse and human adipocytes for the analysis of browning and cellular heterogeneity. Cell Rep. 24(10), 2746-2756 (2018).