Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes[1]. Blasticidin S HCl specifically inhibits protein synthesis in prokaryotic or eukaryotic cells by interfering with ribosome binding peptides. The inhibitory effect of Blasticidin S HCl on translation termination is conserved in eukaryotes and bacteria[2-4].
Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells, Blasticidin S HCl inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production[5]. The presence of Blasticidin S HCl protects DNA from Cu(II) -induced damage[6].
References:
[1]. TAKEUCHI S, HIRAYAMA K, et,al. Blasticidin S, a new antibiotic. J Antibiot (Tokyo). 1958 Jan;11(1):1-5. PMID: 13525246.
[2]. Svidritskiy E, Korostelev AA. Mechanism of Inhibition of Translation Termination by Blasticidin S. J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007. PMID: 29366636; PMCID: PMC5831496.
[3]. Kinoshita T, Tanaka N, et,al. Binding of blasticidin S to ribosomes. J Antibiot (Tokyo). 1970 Jun;23(6):288-90. doi: 10.7164/antibiotics.23.288. PMID: 4917792.
[4]. Svidritskiy E, Ling C, et,al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12283-8. doi: 10.1073/pnas.1304922110. Epub 2013 Jul 3. PMID: 23824292; PMCID: PMC3725078.
[5]. Powers KT, Stevenson-Jones F, et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960.
[6]. Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem. 2013 Oct;127:73-8. doi: 10.1016/j.jinorgbio.2013.06.014. Epub 2013 Jun 29. PMID: 23880034.
Blasticidin S HCl是一种从灰色链霉菌中分离的核苷类抗生素[1]。Blasticidin S HCl通过干扰核糖体结合肽段而特异性抑制原核细胞或真核细胞的蛋白合成。Blasticidin S HCl对翻译终止的抑制作用在真核生物和细菌中是保守的[2-4]。
Blasticidin S HCl处理能够稳定HeLa细胞中过早终止密码子突变的mRNA, Blasticidin S HCl抑制无意义介导的mRNA衰变，并且在亚抑制浓度下，调节过早终止密码子的翻译动力学，从而增强蛋白质产生[5]。Blasticidin S HCl的存在可以保护DNA免受Cu(II)诱导的损伤[6]。