Biotin-PEG4-NHS ester

Biotin-PEG4-NHS ester is a highly hydrophilic amine-reactive biotinylation reagent, which is mainly used for simple and effective biotinylation labeling of antibodies, proteins and other macromolecules containing primary amines (-NH2)^{[1, 2]}. Biotin-PEG4-NHS ester is composed of biotin, polyethylene glycol and N-hydroxysuccinimide ester. Biotin can play a coenzyme function and participate in a variety of carboxylation reactions. Polyethylene glycol makes the biotinylated molecules water-soluble. N-hydroxysuccinimide ester reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds^{[3, 4]}. Biotin-PEG4-NHS ester has good biocompatibility and can be used to prepare biomolecular probes to achieve visualization, positioning and tracking of biomolecules^[5]. Biotin-PEG4-NHS ester can load the enzyme onto super streptavidin (SSA) biosensor^[6].

References:
[1] Shtylev G F, Shishkin I Y, Shershov V E, et al. Immobilization of protein probes on biochips with brush polymer cells[J]. Russian Journal of Bioorganic Chemistry, 2024, 50(5): 2036-2049.
[2]Beard H A. Affinity-guided chemical probes for the study of protein interactions[D]. University of Leeds, 2018.
[3]Sapsford K E, Algar W R, Berti L, et al. Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology[J]. Chemical reviews, 2013, 113(3): 1904-2074.
[4]Jiang H, D'Agostino G D, Cole P A, et al. Selective protein N-terminal labeling with N-hydroxysuccinimide esters[M]//Methods in enzymology. Academic Press, 2020, 639: 333-353.
[5]Xiang Y, Lan T, Lu Y. Using the widely available blood glucose meter to monitor insulin and HbA1c[J]. Journal of Diabetes Science and Technology, 2014, 8(4): 855-858.
[6]Bustad H J, Toska K, Schmitt C, et al. A pharmacological chaperone therapy for acute intermittent porphyria[J]. Molecular Therapy, 2020, 28(2): 677-689.

Biotin-PEG4-NHS ester是一种高亲水性的胺反应性生物素化试剂，主要用于对抗体、蛋白质和其他含伯胺(-NH₂) 的大分子进行简单有效的生物素化标记^{[1, 2]}。Biotin-PEG4-NHS ester由生物素、聚乙二醇和N-羟基琥珀酰亚胺酯组成，生物素能够发挥辅酶功能参与多种羧化反应，聚乙二醇化使生物素化分子具有水溶性，N-羟基琥珀酰亚胺酯与赖氨酸和N端氨基基团发生特异性高效反应形成稳定的酰胺键^{[3, 4]}。Biotin-PEG4-NHS ester具有良好的生物相容性能够用于制备生物分子探针，实现生物分子的可视化、定位和追踪^[5]。Biotin-PEG4-NHS ester能够将酶加载到超级链霉亲和素（SSA）生物传感器上^[6]。