Biotin-PEG3-azide is the PEGified form of biotin-azide and can be used in the synthesis of PROTACs [1]. Biotin-PEG3-azide can be used to label the terminal alkyne through a copper-catalyzed click reaction or an uncopper click reaction with cyclooctyne [2]. Biotin-PEG3-azide can be used in non-enzymatic reactions and covalent labeling of proteins, and can be separated and detected by common laboratory methods such as SDS-PAGE and Western blotting [3].
References:
[1] Flaxman H A. Structural and mechanistic studies of molecular glues and their targets[D]. Harvard University, 2022.
[2] Ge C, Chen X, Wang D. An array of femtoliter wells for sensitive detection of copper using click chemistry[J]. Talanta, 2024, 274: 125973.
[3] Zheng J, Mao Y, Feng S, et al. Combining Metabolic Alkyne Labeling and Click Chemistry for Secretome Analysis of Serum‐Containing Conditioned Medium[J]. Chinese Journal of Chemistry, 2021, 39(7): 1843-1848.
Biotin-PEG3-azide是生物素-叠氮化物的PEG化形式，可用于PROTAC的合成 [1]。Biotin-PEG3-azide可用于通过与环辛炔的铜催化点击反应或无铜点击反应标记末端炔 [2]。Biotin-PEG3-azide可用于非酶促反应和共价标记蛋白质，可以使用常见的实验室方法如SDS-PAGE和Western印迹进行分离和检测 [3]。