Beta-Sitosterol

Cell lines

MH7A cells

Preparation Method

The test was divided into the control group (DMSO), positive drug group (methotrexate, 80μM), and drug intervention group (Beta-Sitosterol, 10, 20, 40, 80, and 160μM, respectively). After cells were treated with different concentrations of Beta-Sitosterol, the MTT method was used to detect the proliferation of cells at 12, 24, and 48h. 

Reaction Conditions

10, 20, 40, 80, 160μM; 12, 24, 48h

Applications

Beta-Sitosterol inhibits the proliferation of MH7A cells in a concentration- and time-dependent manner.

Animal models

Wistar rats

Preparation Method

Used 30 rats organized in 6 groups with 5 individuals each: a negative control group that was intra-gastrically (IG) administered with mineral oil; three other groups that were IG administered with Beta-Sitosterol (50, 100 and 200mg/kg), previously diluted in mineral oil; and finally, two more groups intraperitoneally (i.p.) administered with known anti-inflammatory drugs, in which one group was injected 200mg/kg of ibuprofen (a non-steroidal anti-inflammatory agent), and the other group was injected with 10mg/kg of prednisone (a steroidal anti-inflammatory agent). All animals were immediately injected with 0.1mL of anti-ovalbumin antibody (diluted 1:3) in the hindquarter of the plantar area of the right leg, and in the contralateral leg; rats were injected with 0.1mL of 0.9% NaCl. In a subsequent step, all animals were intravenously (i.v.) injected with a suspension of egg albumin (25mg/kg). The volume of the swollen leg was determined with a digital pletysmometer 3h after the ovoalbumin injection. The paw edema value was established by determining the difference between the volume obtained in the treated leg and in the control (left leg). For data calculation, the volume of the swollen leg was considered to represent 100% inflammation.

Dosage form

50, 100, 200mg/kg; i.p.

Applications

The three tested doses of Beta-Sitosterol were found to induce a significant, dose dependent inhibition of the edema in comparison with the result found in the control group. The inhibition was 51, 63, and 70% with 50, 100, and 200mg/kg of Beta-Sitosterol, respectively.

References:
[1]Wang X, Mao J. Systematic pharmacology-based strategy to investigate the mechanism of beta-sitosterol for the treatment of rheumarthritis[J]. Frontiers in Genetics, 2024, 15: 1507606.
[2]Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, et al. Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(1): 123-130.