BCA (Disodium bicinchoninate) is a colorimetric reagent for the detection of copper [1]. BCA is stable under alkali conditions, and can react with Cu+ (2:1) to form an intense purple complex, which has a peak absorbance at 562nm [2]. BCA has been widely used for detecting protein concentrations and for quantitative analysis of the number of anchoring-dependent cells[3].
References:
[1] Wiechelman K J, Braun R D, Fitzpatrick J D. Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation[J]. Analytical biochemistry, 1988, 175(1): 231-237.
[2] Huang T, Long M, Huo B. Competitive binding to cuprous ions of protein and BCA in the bicinchoninic acid protein assay[J]. The open biomedical engineering journal, 2010, 4: 271.
[3] Tuszynski G P, Murphy A. Spectrophotometric quantitation of anchorage-dependent cell numbers using the bicinchoninic acid protein assay reagent[J]. Analytical biochemistry, 1990, 184(1): 189-191.
BCA (Disodium bicinchoninate)是一种用于检测铜的比色试剂[1]。BCA在碱性条件下稳定，可与Cu+以2:1的比例反应形成深紫色复合物，在562nm处具有最大吸收峰[2]。BCA已被广泛用于检测蛋白质浓度以及对贴壁定依赖性细胞数量进行定量分析[3]。