AF488 NHS ester is an amine-specific fluorescent probe with optimal excitation wavelength of 488nm and the emission wavelength of 525nm[1]. AF488 NHS ester is pH-sensitive and emits fluorescence at low pH values, which can be used to detect the entry of nanoparticles into lysosomes with low pH levels[2]. AF488 NHS ester can be used to label viruses for tracking virus-cell interactions[3]. AF488 NHS ester can be used to prepare antibodies against Staphylococcus aureus(S. aureus), thereby establishing a S. aureus detection method based on fluorescence immunosorbent assay[4].
References:
[1] Kendall C G, Stockton A M, Leicht S, et al. Amine analysis using AlexaFluor 488 succinimidyl ester and capillary electrophoresis with laser‐induced fluorescence[J]. Journal of Analytical Methods in Chemistry, 2015, 2015(1): 368362.
[2] Vaughan H J, Est-Witte S, Dockery L T, et al. A high-throughput lysosome trafficking assay guides ligand selection and elucidates differences in CD22-targeted nanodelivery[J]. Science and Technology of Advanced Materials, 2024, 25(1): 2351791.
[3] Zhang S L X, Tan H C, Hanson B J, et al. A simple method for Alexa Fluor dye labelling of dengue virus[J]. Journal of virological methods, 2010, 167(2): 172-177.
[4] Kim J K, Yun H Y, Kim J S, et al. Development of fluorescence-linked immunosorbent assay for rapid detection of Staphylococcus aureus[J]. Applied Microbiology and Biotechnology, 2024, 108(1): 2.
AF488 NHS ester是一种胺特异性荧光探针，最佳激发波长为488nm，发射波长为525nm[1]。AF488 NHS ester具有pH敏感性，在低pH值时发出荧光，可用于检测纳米颗粒进入低pH值溶酶体的过程[2]。AF488 NHS ester可用于标记病毒，以追踪病毒与细胞的相互作用[3]。AF488 NHS ester可用于制备针对金黄色葡萄球菌（S. aureus）的抗体，从而建立基于荧光免疫吸附法的金黄色葡萄球菌检测方法[4]。