Adrenomedullin (AM) (1-52), human is a biologically active peptide composed of 52 amino acids, belonging to the calcitonin gene-related peptide superfamily, and exhibits significant vasodilatory effects[1-2]. Adrenomedullin (AM) (1-52), human acts as a regulatory factor for the growth of adult neural stem cells, influencing their proliferation rate and differentiation fate into neurons, astrocytes, and oligodendrocytes[3]. Adrenomedullin (AM) (1-52), human can modulate the proliferation process of cancer cells and promote angiogenesis, playing an important role in both cancer development and vascular formation during tissue repair[4].
In vitro, pretreatment of H9c2 cardiomyocytes with Adrenomedullin (AM) (1-52), human (10nM) for 30 minutes, followed by stimulation with palmitic acid (200μM) for 24 hours, significantly suppressed the protein expression of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, while reducing intracellular reactive oxygen species levels and improving cell viability[5]. In hypoxically cultured bovine articular chondrocytes, pretreatment with Adrenomedullin (AM) (1-52), human (100nM) for 30 minutes, followed by stimulation with Fas ligand (20ng/mL) for 24 hours, significantly inhibited the activity of caspase-3 and caspase-8, while reducing the apoptosis rate[6].
In vivo, continuous infusion of Adrenomedullin (AM) (1-52), human (0.05mg/kg/h) via a carotid artery catheter in a C57/BL6 mouse model of lung injury induced by high tidal volume (17ml/kg) mechanical ventilation for 2 hours or moderate tidal volume (12ml/kg) ventilation for 6 hours, significantly reduced ventilator-induced increases in pulmonary vascular permeability and improved oxygenation function[7]. Continuous subcutaneous infusion of Adrenomedullin (AM) (1-52), human (0.05mg/kg/min) via an osmotic minipump in BALB/c mice treated with a single intraperitoneal injection of doxorubicin (20mg/kg) significantly improved the 14-day survival rate of mice exposed to high-dose doxorubicin and reduced serum lactate dehydrogenase levels[8].
References:
[1] Martínez-Herrero S, Martínez A. Adrenomedullin: Not Just Another Gastrointestinal Peptide. Biomolecules. 2022 Jan 18;12(2):156.
[2] Ferrero H, Larrayoz IM, Gil-Bea FJ, et al. Adrenomedullin, a Novel Target for Neurodegenerative Diseases. Mol Neurobiol. 2018 Dec;55(12):8799-8814.
[3] Nishikimi T, Kinoshita H, Inazumi H, et al. Adrenomedullin in pulmonary hypertension. Dan Med J. 2024 Nov 15;71(12):A07240498.
[4] Li H, Yang W, Wang S, et al. Adrenomedullin in Tumorigenesis and Cancer Progression. Int J Mol Sci. 2025 Jun 10;26(12):5552.
[5] Qian P, Wang Q, Wang FZ, et al. Adrenomedullin Improves Cardiac Remodeling and Function in Obese Rats with Hypertension. Pharmaceuticals (Basel). 2022 Jun 6;15(6):719.
[6] Velard F, Chatron-Colliet A, Côme D, et al. Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death. Sci Rep. 2020 Oct 7;10(1):16740.
[7] Müller HC, Witzenrath M, Tschernig T, et al. Adrenomedullin attenuates ventilator-induced lung injury in mice. Thorax. 2010 Dec;65(12):1077-84.
[8] Yoshizawa T, Takizawa S, Shimada S, et al. Effects of Adrenomedullin on Doxorubicin-Induced Cardiac Damage in Mice. Biol Pharm Bull. 2016 May 1;39(5):737-46.
Adrenomedullin (AM) (1-52), human是一种由52个氨基酸组成的生物活性多肽，属于降钙素基因相关肽超家族，具有显著的血管舒张作用[1-2]。Adrenomedullin (AM) (1-52), human能作为成年神经干细胞生长的调节因子，影响其增殖速率以及向神经元、星形胶质细胞和少突胶质细胞的分化命运[3]。Adrenomedullin (AM) (1-52), human能够影响癌细胞的增殖过程并促进血管生成，在癌症发展和组织修复的血管形成中扮演重要角色[4]。
在体外，Adrenomedullin (AM) (1-52), human (10nM) 预处理H9c2心肌细胞30分钟，随后以棕榈酸 (200μM) 刺激24小时，Adrenomedullin (AM) (1-52), human显著抑制促炎因子TNF-α、IL-1β和IL-6的蛋白表达，同时降低细胞内活性氧水平并改善细胞活力[5]。Adrenomedullin (AM) (1-52), human（100nM）预处理缺氧培养的牛关节软骨细胞30分钟，随后以Fas配体（20ng/mL）刺激24小时，Adrenomedullin (AM) (1-52), human显著抑制caspase-3和caspase-8的活性，同时降低细胞凋亡率[6]。
在体内，Adrenomedullin (AM) (1-52), human (0.05mg/kg/h) 通过颈动脉导管持续输注，用于处理接受高潮气量(17ml/kg)机械通气2小时或中等潮气量(12ml/kg)通气6小时诱导的肺损伤C57/BL6小模型，Adrenomedullin (AM) (1-52), human显著降低了呼吸机诱导的肺血管通透性增高，同时改善了氧合功能[7]。Adrenomedullin (AM) (1-52), human (0.05mg/kg/min) 通过渗透性微型泵持续皮下输注，用于处理单次腹腔注射20mg/kg阿霉素的BALB/c小鼠，Adrenomedullin (AM) (1-52), human显著提高了高剂量阿霉素处理小鼠的14天生存率，同时降低了血清乳酸脱氢酶水平[8]。