Acridine Orange hydrochloride is a cell-permeable, nucleic-acid-selective fluorescent dye. When bound to nucleic acids, Acridine Orange hydrochloride emits fluorescence: upon intercalation into double-stranded DNA produces green fluorescence (excitation 488nm, emission 530nm). Because Acridine Orange hydrochloride associates with DNA and RNA to different extents, Acridine Orange hydrochloride binding to single-stranded DNA or RNA yields orange to red fluorescence (excitation 488nm, emission 640nm). Consequently, Acridine Orange hydrochloride is commonly employed to distinguish normal, apoptotic, and necrotic cells[1].
References：
[1] Ma T, Zhao Y, Cheng X. Detection of dsRNA by Acridine Orange hydrochloride Staining. Methods Mol Biol. 2024;2771:7-12.
Acridine Orange hydrochloride是一种可穿透细胞的核酸选择性荧光染料，Acridine Orange hydrochloride可与DNA或RNA结合时产生荧光，与双链DNA结合时产生绿色荧光（激发光为488nm；发射光为530nm绿色荧光）。Acridine Orange hydrochloride与细胞中DNA和RNA结合量存在差别，Acridine Orange hydrochloride与单链DNA或RNA结合量结合量多发桔黄色或桔红色荧光（激发光为488nm；发射光为640nm）。因此，常被用于鉴别正常细胞、凋亡细胞及坏死细胞[1].。