Ac-PAL-AMC is a fluorescent substrate that is specific to the 20S proteasome LMP2/β1i subunit. The maximum excitation/emission wavelength of the fluorophore 7-amino-4-methylcoumarin (AMC) is 380nm/460nm[1, 2, 3].
References:
[1] Erokhov P A, Kulikov A M, Karpova Y D, et al. Proteasomes in Patient Rectal Cancer and Different Intestine Locations: Where Does Proteasome Pool Change?. Cancers 2021, 13, 1108[EB/OL].(2021)
[2] Obayashi Y, Suzuki S. Proteolytic enzymes in coastal surface seawater: significant activity of endopeptidases and exopeptidases[J]. Limnology and oceanography, 2005, 50(2): 722-726.
[3] Schulze A, Wermann M, Demuth H U, et al. Continuous assays for meprin alpha and beta using prolyl tripeptidyl aminopeptidase (PtP) from Porphyromonas gingivalis[J]. Analytical biochemistry, 2018, 559: 11-16.
Ac-PAL-AMC是一种荧光底物，是20S蛋白酶体LMP2/β1i亚基的特异性荧光底物，荧光团7-amino-4-methylcoumarin（AMC）的最大激发/发射波长为380nm/460nm[1, 2, 3]。