β-Amyrone (β-Amyron) is a triterpene compound which has anti-inflammatory activity through inhibiting the expression of COX-2. β-Amyrone has antifungal activity , as well as antiviral activity against Chikungunya virus. β-Amyrone also inhibits α-glucosidase and acetylcholinesterase (AChE) activity. β-Amyrone can be used in the research of disease like inflammation, infection, and obesity[1][2][3][4].
β-Amyrone (1.25-10 μg/mL, 24 h) inhibits on NO? production in LPS-stimulated J774 cells, with an IC50 value of 4.61 μg/mL, and has no obvious effect on cell viability[1].
β-Amyrone (10 μg/mL, 24 h) inhibits IL-6, IL-10 levels, and COX-2 expression in LPS-stimulated J774 cells[1].
β-Amyrone (0-235 μM, 7days) has antiviral activity in Vero cells against Chikungunya virus (CHIKV), with EC50 value of 86 uM[2].
β-Amyrone inhibits α-glucosidase, acetylcholinesterase (AChE) and fungal activity with IC50 values of 25 μM, 23 μM and 8 μg/mL, respectively[4].
Western Blot Analysis[1]
| Cell Line: | LPS (1 μg/mL)-stimulated J774 cells |
| Concentration: | 2.5, 5, 10 μg/mL |
| Incubation Time: | 24 h |
| Result: | Inhibited COX-2 expression in a concentration-dependent manner (approximately 90% reduction at 5 or 10 μg/ mL). |
β-Amyrone (local administration on ear, 0.1-0.6 mg/kg, a single dose) inhibits ear edema formation in phenol-induced edema mice[1].
| Animal Model: | Ear phenol-induced edema in Balb C mice[1] |
| Dosage: | 0.1, 0.3, 0.6 mg/kg, a single dose |
| Administration: | Local administration (20 ??L solution) on ear |
| Result: | Inhibited ear edema formation in a dose-related manner (47% inhibition at the dose of 0.6mg/kg). |