8-O-Acetylharpagide is an iridoid compound isolated from Ajuga reptile. The compound has antibacterial, antispasmodic, cardiotonic and antipyretic properties[1] [2].
8-O-Acetylharpagide (1-10μM; 24h) treatment inhibited ROS in a concentration-dependent manner in old HDFs. Low concentrations (less than 10μM) of 8-O-Acetylharpagide (5-50μg/ml; 48h) can fully inhibit the senescence-associated phenotypic expression of p53 and SA-β-gal in aged HDFs[1]. Different concentrations of 8-O-Acetylharpagide (100-250μg/ml; 24h) were active against Bacillus anthracis, but inactive against Staphylococcus aureus, Streptococcus agalactiae, Staphylococcus epidermidis, Aeromonas sobria, Shigella boydii, Vibrio cholerae, Corynebacterium diphtheriae, Salmonella typhi (Gram-positive bacteria), and Pseudomonas aeruginosa (Gram-positive bacteria) [2].
8-O-Acetylharpagide (100-1000µg/ml; immersion 20s) showed spasmolytic effects on isolated ileum and cardiotonic effects on isolated rabbit hearts at a test dose of 100µg/ml. No harmful or toxic effects of 8-O-Acetylharpagide (1-100 μg; po; 1 time) were detected[2].
References:
[1].Shafi N, Khan G A, Arfan M, et al. Isolation and pharmacological screening of 8-O-acetyl harpagide from Ajuga bracteosa Wall[J]. Biological Sciences-PJSIR, 2004, 47(3): 176-179.
[2].Hsu W H, Lin B Z, Leu J D, et al. Involvement of 8-O-acetylharpagide for Ajuga taiwanensis mediated suppression of senescent phenotypes in human dermal fibroblasts[J]. Scientific reports, 2020, 10(1): 19731.
8-O-Acetylharpagide是从Ajuga属爬行动物中分离的一种环烯醚萜类化合物。该化合物具有抗菌、解痉、强心和解热作用[1] [2]。
8-O-Acetylharpagide（1-10μM；24h）以浓度依赖性方式抑制老年HDF中的ROS。低浓度（小于10μM）的8-O-Acetylharpagide（5-50μg/ml；48h）可以完全抑制老年HDF中p53和SA-β-半乳糖苷酶的衰老相关表型表达[1]。不同浓度的8-O-Acetylharpagide（100-250μg/ml；24h）对炭疽芽孢杆菌有活性，但对金黄色葡萄球菌、无乳链球菌、表皮葡萄球菌、温和气单胞菌、鲍氏志贺氏菌、霍乱弧菌、白喉棒状杆菌、伤寒沙门氏菌（革兰氏阳性菌）和铜绿假单胞菌（革兰氏阳性菌）无效[2]。
8-O-Acetylharpagide（100-1000µg/ml；浸泡20s）在 100µg/ml 测试剂量下对离体回肠表现出解痉作用，对离体兔心脏表现出强心作用。未检测到 8-O-Acetylharpagide (1-100μg；po；1 次) 的有害或毒性作用[2]。